Abstract |
The present study was aimed to investigate the effect of 28-homobrassinolide on leukotriene synthesis in the 2H3 cells. Cell viability was determined by using sulforhodamine B (SRB) assay. Leukotriene C4 ( LTC4) and Leukotriene B4 ( LTB4) were determined in the cell and cell lysates. Intracellular Ca2+ was determined in the intact cells. Phospholipases A2 (PLA2) expression was determined in the cells using immunofluorescence. LTC4 and LTB4 were tremendously increased in IgE-loaded 2H3 cells. However, these levels were significantly reduced following treatment with 28-homobrassinolide. The intracellular Ca2+ level was not altered by treatment. Expression of PLA2 was significantly reduced following treatment with 28-homobrassinolide. Taking all these data together, it is suggested that the 28-homobrassinolide may be a potential therapeutic agent to inhibit leukotriene synthesis in 2H3 cells.
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Authors | Chuan He, Zhigang Liu, Jingcao Huang, Ting Liu |
Journal | Cytotechnology
(Cytotechnology)
Vol. 70
Issue 1
Pg. 431-438
(Feb 2018)
ISSN: 0920-9069 [Print] United States |
PMID | 29101583
(Publication Type: Journal Article)
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