Abstract | BACKGROUND: Enterococcus faecalis 2001 is a probiotic lactic acid bacterium and has been used as a biological response modifier (BRM). From physiological limitation of bacterial preservation in storage and safety, the live E. faecalis 2001 has been heat-treated and the BRM components containing high level of β- glucan, named EF-2001, were prepared. METHOD: The heat-treated EF-2001 has been examined for the antioxidative potential for radical scavenging and anti- tumor activities as well as immune-enhancing response in mice. Lymphocyte versus polymorphonuclear leukocyte ratio was increased in mice upon treatment with EF-2001. The number of lymphocytes was increased in the EF-2001-treated group. In the mice bearing two different Ehrlich solid and Sarcoma-180 carcinomas, the treatment with EF-2001 resulted in anti- tumor action. Tumor-suppressive capacity upon treatment with EF-2001 was significantly increased compared to normal controls. RESULTS: During the time interval administration of 5 weeks between the priming and secondary administration of EF-2001, the expression and production levels of TNF-α were also observed in the EF- 2001-administered mice. Additionally, anti- tumor activity examined with the intravenous administration of EF 2001 with a 34 times interval was also observed, as the growth of Sarcoma180 cells was clearly inhibited by the EF-2001. CONCLUSION: From the results, it was suggested that the immune response is enhanced due to antioxidative activity caused by the EF-2001 and anti- tumor activity by NK cells and TNF-α.
|
Authors | Yeun-Hwa Gu, Hyunju Choi, Takenori Yamashita, Ki-Mun Kang, Masahiro Iwasa, Moon-Jo Lee, Kyoung Hae Lee, Cheorl-Ho Kim |
Journal | Current pharmaceutical biotechnology
(Curr Pharm Biotechnol)
Vol. 18
Issue 8
Pg. 653-661
(Nov 10 2017)
ISSN: 1873-4316 [Electronic] Netherlands |
PMID | 28969561
(Publication Type: Journal Article)
|
Copyright | Copyright© Bentham Science Publishers; For any queries, please email at [email protected]. |
Chemical References |
- Antineoplastic Agents
- Antioxidants
- Immunologic Factors
- Tumor Necrosis Factor-alpha
- beta-Glucans
|
Topics |
- Animals
- Antineoplastic Agents
(isolation & purification, pharmacology)
- Antioxidants
(isolation & purification, pharmacology)
- Carcinoma, Ehrlich Tumor
(drug therapy, immunology)
- Enterococcus faecalis
- Immunologic Factors
(isolation & purification, pharmacology)
- Killer Cells, Natural
(drug effects)
- Lymphocytes
(drug effects, immunology)
- Macrophages
(drug effects, immunology)
- Male
- Mice, Inbred ICR
- Neutrophils
(drug effects, immunology)
- Probiotics
- Sarcoma 180
(drug therapy, immunology)
- Tumor Necrosis Factor-alpha
(analysis)
- beta-Glucans
(isolation & purification, pharmacology)
|