Abstract | BACKGROUND:
MiRNAs may be associated with the risk of uterine sarcoma and related molecular mechanism remains unclear. METHODS: A total of 101 patients with uterine sarcoma (cases) and 54 healthy subjects (controls) were enrolled. The levels of serum miR-152, miR-205, miR-222, miR-24, miR-150 and sirtuin 1 ( SIRT1, an NAD +-dependent class III histone deacetylase) were measured by qRT-PCR. HeLa cells were transfected with the mimics of miR-152 and miR-24. The autophagic rates, and the levels of SIRT1 and acetylation of microtubule-associated protein 1A/1B-light chain 3 (LC3) were measured. RESULTS: Levels of miR-152, miR-24 and SIRT1 decreased while the levels of miR-205, miR-222 and miR-150 increased in cases vs. controls (all P < 0.05). All miRNAs were linked with stage of the cases' sarcoma (all P = 0.001). Kaplan-Meier analysis demonstrated uterine sarcoma patients have better survival rates with high-level miR-152 and miR-24, with a five-year overall survival of 21.8% and 67.5%, respectively (P = 0.003 and 0.004). The mimics of miR-152 and miR-24 induced autophagy by increasing the level of SIRT1, which deacetylated LC3. CONCLUSION: Present findings demonstrate altered miRNA species in uterine sarcoma that are linked to disease stage, and a new molecular mechanism, by which miR-152 and miR-24 promote autophagy by activating SIRT1 and deacetylating LC3.
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Authors | X Tong, X Wang, C Wang, L Li |
Journal | British journal of biomedical science
(Br J Biomed Sci)
Vol. 75
Issue 1
Pg. 7-12
(Jan 2018)
ISSN: 0967-4845 [Print] Switzerland |
PMID | 28929922
(Publication Type: Journal Article)
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Chemical References |
- MAP1LC3A protein, human
- MIRN152 microRNA, human
- MIRN24 microRNA, human
- MicroRNAs
- Microtubule-Associated Proteins
- Oligodeoxyribonucleotides
- SIRT1 protein, human
- Sirtuin 1
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Topics |
- Acetylation
- Adult
- Autophagy
(genetics)
- Case-Control Studies
- Female
- Gene Expression Regulation, Neoplastic
- HeLa Cells
- Humans
- Kaplan-Meier Estimate
- MicroRNAs
(antagonists & inhibitors, genetics, metabolism)
- Microtubule-Associated Proteins
(genetics, metabolism)
- Middle Aged
- Neoplasm Staging
- Oligodeoxyribonucleotides
(genetics, metabolism)
- Sarcoma
(genetics, metabolism, mortality, pathology)
- Signal Transduction
- Sirtuin 1
(genetics, metabolism)
- Transfection
- Uterine Cervical Neoplasms
(genetics, metabolism, mortality, pathology)
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