Osteogenesis for postoperative temporal bone defects using human ear adipose-derived stromal cells and tissue engineering: An animal model study.

Abstract

Mastoidectomy, the removal of infected mastoid bones, is a common surgical procedure for the treatment of chronic otitis media. Persistent and recurrent otorrhea and accumulation of keratin debris following open cavity mastoidectomy are still bothersome issues for both patients and otologists. In this study, we used human ear adipose-derived stromal cells (hEASCs) in combination with polycaprolactone (PCL) scaffolds and osteogenic differentiation medium (ODM) to regenerate temporal bone defects. The hEASCs showed stem cell phenotypes, and these characteristics were maintained up to passage 5. Mastoid bulla and cranial bone defects were induced in Sprague-Dawley rats using AgNO3 and burr hole drilling, respectively, and the rats were then divided into five groups: (1) control, (2) hEASCs, (3) hEASCs + ODM, (4) hEASCs + PCL scaffolds, and (5) hEASCs + PCL scaffolds + ODM. Osteogenesis was evaluated by micro-computed tomography and histology. Compared with the control group, the groups transplanted with hEASCs and PCL scaffolds had significantly higher bone formation along the periphery of the mastoid bulla area. Moreover, ODM synergistically enhanced bone formation in mastoid bulla defects. Our results suggest that combining hEASCs with PCL scaffolds represents a promising method for anatomical and functional reconstruction of postoperative temporal bone defects following mastoidectomy. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3493-3501, 2017.

• Authors: Yeon Ju Kim, Seung Gu Park, Beomyong Shin, Jangho Kim, Seung Won Kim, Oak-Sung Choo, Xiang Yun Yin, Byoung Hyun Min, Yun-Hoon Choung
• Journal: Journal of biomedical materials research. Part A (J Biomed Mater Res A) Vol. 105 Issue 12 Pg. 3493-3501 (Dec 2017) ISSN: 1552-4965 [Electronic] United States
• PMID: 28875515 (Publication Type: Journal Article)
• Copyright: © 2017 Wiley Periodicals, Inc.

Chemical References

• Polyesters
• polycaprolactone

Topics

• Adipose Tissue (cytology)
• Animals
• Bone Regeneration
• Cells, Cultured
• Humans
• Male
• Osteogenesis
• Polyesters (chemistry)
• Rats, Sprague-Dawley
• Skull (injuries, pathology, physiology)
• Stromal Cells (cytology, transplantation)
• Tissue Engineering (methods)
• Tissue Scaffolds (chemistry)