Protective role of humic acids against picloram-induced genomic instability and DNA methylation in Phaseolus vulgaris.

Abstract

Picloram (4-amino-3,5,6-trichloropicolinic acid) is a liquid auxinic herbicide used to control broad-leaved weeds. Picloram is representing a possible hazard to ecosystems and human health. Therefore, in this study, DNA methylation changes and DNA damage levels in Phaseolus vulgaris exposed to picloram, as well as whether humic acid (HA) has preventive effects on these changes were investigated. Random amplified polymorphic DNA (RAPD) techniques were used for identification of DNA damage and coupled restriction enzyme digestion-random amplification (CRED-RA) techniques were used to detect the changed pattern of DNA methylation. According to the obtained results, picloram (5, 10, 20, and 40 mg/l) caused DNA damage profile changes (RAPDs) increasing, DNA hypomethylation and genomic template stability (GTS) decreasing. On the other hand, different concentrations of applied HA (2, 4, 6, 8, and 10%) reduced hazardous effects of picloram. The results of the experiment have explicitly indicated that HAs could be an alternative for reducing genetic damage in plants. In addition to the alleviate effects of humic acid on genetic damage, its epigenetic effect is hypomethylation.

• Authors: Mahmut Sinan Taspinar, Murat Aydin, Burcu Sigmaz, Nalan Yildirim, Guleray Agar
• Journal: Environmental science and pollution research international (Environ Sci Pollut Res Int) Vol. 24 Issue 29 Pg. 22948-22953 (Oct 2017) ISSN: 1614-7499 [Electronic] Germany
• PMID: 28819832 (Publication Type: Journal Article)

Chemical References

• Herbicides
• Humic Substances
• Picloram

Topics

• DNA Damage
• DNA Methylation (drug effects)
• Dose-Response Relationship, Drug
• Genomic Instability (drug effects)
• Herbicides (toxicity)
• Humic Substances (analysis)
• Models, Theoretical
• Phaseolus (drug effects, genetics)
• Picloram (toxicity)
• Random Amplified Polymorphic DNA Technique