HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Interleukin-13/interleukin-4 receptor pathway is crucial for production of Sda-sialomucin in mouse small intestinal mucosa by Nippostrongylus brasiliensis infection.

Abstract
Mucin is a major component of mucus in gastrointestinal mucosa. Increase of specific sialomucins having Sda blood group antigen, NeuAcα2-3(GalNAcβ1-4)Galβ1-4GlcNAcβ-, is considered to be associated with expulsion of the parasitic intestinal nematode Nippostrongylus brasiliensis. In this study, we examined the relationship between interleukin (IL)-13 pathway and expression of Sda-sialomucins in small intestinal mucosa with N. brasiliensis infection. Nematode infection induced marked increases in small intestinal mucins that reacted with anti-Sda antibody in wild type (wt) mice. However, this increase due to infection was supressed in IL-4 receptor α deficient (IL-4Rα-/-) mice, which lack both IL-4 and IL-13 signaling via IL-4R, and severe combined immunodeficient (SCID) mice, which have defects in B- and T-lymphocytes. Analysis using tandem mass spectroscopy showed that Sda-glycans were not expressed in small intestinal mucins in IL-4Rα-/- and SCID mice after infection despite the appearance of Sda-glycans in the infected wt mice. Inoculation of recombinant IL-13 into the infected SCID mice restored expression of Sda-glycan. Our results suggest that the IL-13/IL-4R axis is important for the production of Sda-sialomucins in the host intestinal mucosa with parasitic nematode infection.
AuthorsDaigo Tsubokawa, Kenji Ishiwata, Yukinobu Goso, Takeshi Nakamura, Takeshi Hatta, Kazuhiko Ishihara, Hirotaka Kanuka, Naotoshi Tsuji
JournalParasitology international (Parasitol Int) Vol. 66 Issue 6 Pg. 731-734 (Dec 2017) ISSN: 1873-0329 [Electronic] Netherlands
PMID28802865 (Publication Type: Journal Article)
CopyrightCopyright © 2017 Elsevier B.V. All rights reserved.
Chemical References
  • Receptors, Interleukin-4, Type II
  • Sialomucins
Topics
  • Adaptive Immunity
  • Animals
  • Intestinal Diseases, Parasitic (immunology, parasitology)
  • Intestinal Mucosa (immunology)
  • Intestine, Small (immunology)
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, SCID
  • Nippostrongylus (physiology)
  • Receptors, Interleukin-4, Type II (genetics, metabolism)
  • Sialomucins (metabolism)
  • Signal Transduction
  • Strongylida Infections (immunology, parasitology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: