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Ouabain impairs cell migration, and invasion and alters gene expression of human osteosarcoma U-2 OS cells.

Abstract
Ouabain, the specific Na+ /K+ -ATPase blocker, has biological activity including anti-proliferative and anti-metastasis effects in cancer cell. There is no study to show ouabain inhibiting cell migration and invasion in human osteosarcoma U-2 OS cells. Thus, we investigated the effect of ouabain on the cell migration and invasion of human osteosarcoma U-2 OS cells. Results indicated that ouabain significantly decreased the percentage of viable cells at 2.5-5.0 μM, thus, we selected 0.25-1.0 μM for inhibiting studies. Ouabain inhibited cell migration, invasion and the enzymatic activities of MMP-2, and also affected the expression of metastasis-associated protein in U-2 OS cells. The cDNA microarray assay indicated that CDH1, TGFBR3, SHC3 and MAP2K6 metastasis-related genes were increased, but CCND1, JUN, CDKN1A, TGFB1, 2 and 3, SMAD4, MMP13, MMP2 and FN1 genes were decreased. These findings provide more information regarding ouabain inhibited cell migration and invasion and associated gene expressions in U-2 OS cells after exposed to ouabain.
AuthorsYung-Luen Shih, Man-Kuan Au, Ko-Lin Liu, Ming-Yang Yeh, Ching-Hsiao Lee, Mei-Hui Lee, Hsu-Feng Lu, Jiun-Long Yang, Rick Sai-Chuen Wu, Jing-Gung Chung
JournalEnvironmental toxicology (Environ Toxicol) Vol. 32 Issue 11 Pg. 2400-2413 (Nov 2017) ISSN: 1522-7278 [Electronic] United States
PMID28795476 (Publication Type: Journal Article)
Copyright© 2017 Wiley Periodicals, Inc.
Chemical References
  • Antineoplastic Agents
  • Matrix Metalloproteinase Inhibitors
  • Ouabain
  • Matrix Metalloproteinase 2
  • Sodium-Potassium-Exchanging ATPase
Topics
  • Antineoplastic Agents (pharmacology)
  • Bone Neoplasms
  • Cell Line, Tumor
  • Cell Movement (drug effects)
  • Humans
  • Matrix Metalloproteinase 2 (metabolism)
  • Matrix Metalloproteinase Inhibitors (pharmacology)
  • Neoplasm Invasiveness
  • Osteosarcoma
  • Ouabain (pharmacology)
  • Sodium-Potassium-Exchanging ATPase (antagonists & inhibitors)

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