Abstract |
Ouabain, the specific Na+ /K+ - ATPase blocker, has biological activity including anti-proliferative and anti- metastasis effects in cancer cell. There is no study to show ouabain inhibiting cell migration and invasion in human osteosarcoma U-2 OS cells. Thus, we investigated the effect of ouabain on the cell migration and invasion of human osteosarcoma U-2 OS cells. Results indicated that ouabain significantly decreased the percentage of viable cells at 2.5-5.0 μM, thus, we selected 0.25-1.0 μM for inhibiting studies. Ouabain inhibited cell migration, invasion and the enzymatic activities of MMP-2, and also affected the expression of metastasis-associated protein in U-2 OS cells. The cDNA microarray assay indicated that CDH1, TGFBR3, SHC3 and MAP2K6 metastasis-related genes were increased, but CCND1, JUN, CDKN1A, TGFB1, 2 and 3, SMAD4, MMP13, MMP2 and FN1 genes were decreased. These findings provide more information regarding ouabain inhibited cell migration and invasion and associated gene expressions in U-2 OS cells after exposed to ouabain.
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Authors | Yung-Luen Shih, Man-Kuan Au, Ko-Lin Liu, Ming-Yang Yeh, Ching-Hsiao Lee, Mei-Hui Lee, Hsu-Feng Lu, Jiun-Long Yang, Rick Sai-Chuen Wu, Jing-Gung Chung |
Journal | Environmental toxicology
(Environ Toxicol)
Vol. 32
Issue 11
Pg. 2400-2413
(Nov 2017)
ISSN: 1522-7278 [Electronic] United States |
PMID | 28795476
(Publication Type: Journal Article)
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Copyright | © 2017 Wiley Periodicals, Inc. |
Chemical References |
- Antineoplastic Agents
- Matrix Metalloproteinase Inhibitors
- Ouabain
- Matrix Metalloproteinase 2
- Sodium-Potassium-Exchanging ATPase
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Topics |
- Antineoplastic Agents
(pharmacology)
- Bone Neoplasms
- Cell Line, Tumor
- Cell Movement
(drug effects)
- Humans
- Matrix Metalloproteinase 2
(metabolism)
- Matrix Metalloproteinase Inhibitors
(pharmacology)
- Neoplasm Invasiveness
- Osteosarcoma
- Ouabain
(pharmacology)
- Sodium-Potassium-Exchanging ATPase
(antagonists & inhibitors)
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