Prognostic significance of hMSH2, hMSH3, and hMSH6 expression in ameloblastoma.

Abstract

OBJECTIVE:

The aim of this study was to investigate hMutS proteins in developing human tooth, ameloblastomas, and ameloblastic carcinoma and to determine whether the expression of these proteins has any prognostic potential.

STUDY DESIGN:

Ten cases of developing human tooth, 39 ameloblastomas, and 2 ameloblastic carcinomas were used to determine the distribution of the proteins during the process of carcinogenesis. Simultaneously, another sample of 73 ameloblastomas was arranged in tissue microarray, and their clinical, microscopic, and radiographic features; treatment outcome; presence of BRAF-V600E mutation; and follow-up data were assessed to determine the prognostic relevance of the expression of hMutS (hMSH2, hMSH3, hMSH6) and Ki-67. hMSH2 and hMSH6 were significantly downexpressed in ameloblastomas (P = .0059) compared with developing human tooth (P < .0001).

RESULTS:

hMSH2, hMSH3 expression were significantly associated with BRAF-V600E mutation (P < .05). Simultaneous overexpression of hMutS was associated with recurrence (P = .035); however, these did not predict the disease-free survival of patients (P > .05).

CONCLUSIONS:

hMutS proteins are downregulated in ameloblastoma; moreover, simultaneous overexpression of these proteins in ameloblastoma was associated with recurrence but did not predict disease-free survival.

• Authors: Gleyson Kleber do Amaral-Silva, Celeste Sánchez-Romero, Vivian Petersen Wagner, Manoela Domingues Martins, Hélder Antônio Rebelo Pontes, Eduardo Rodrigues Fregnani, Fernando Augusto Soares, Oslei Paes de Almeida, André Caroli Rocha, Alan Roger Santos-Silva, Felipe Paiva Fonseca, Pablo Agustin Vargas
• Journal: Oral surgery, oral medicine, oral pathology and oral radiology (Oral Surg Oral Med Oral Pathol Oral Radiol) Vol. 124 Issue 3 Pg. 286-295 (Sep 2017) ISSN: 2212-4411 [Electronic] United States
• PMID: 28734697 (Publication Type: Journal Article, Multicenter Study)
• Copyright: Copyright © 2017 Elsevier Inc. All rights reserved.

Chemical References

• DNA-Binding Proteins
• G-T mismatch-binding protein
• MSH3 protein, human
• MutS Homolog 3 Protein
• MSH2 protein, human
• MutS Homolog 2 Protein

Topics

• Adult
• Ameloblastoma (metabolism)
• Brazil
• DNA-Binding Proteins (metabolism)
• Down-Regulation
• Female
• Humans
• Immunohistochemistry
• In Vitro Techniques
• Jaw Neoplasms (metabolism)
• Male
• MutS Homolog 2 Protein (metabolism)
• MutS Homolog 3 Protein (metabolism)
• Prognosis