Identification of trunk mutations in gastric carcinoma: a case study.

Abstract

BACKGROUND:

Intratumor heterogeneity (ITH) poses an urgent challenge for cancer precision medicine because it can cause drug resistance against cancer target therapy and immunotherapy. The search for trunk mutations that are present in all cancer cells is therefore critical for each patient.

CASE PRESENTATION:

In this study, we aimed to evaluate the efficiency of multiregional sequencing for the identification of trunk mutations present in all regions of a tumor as a case study. We applied multiregional whole-exome sequencing (WES) to investigate the genetic heterogeneity and homogeneity of a case of gastric carcinoma. Approximately 83% of common missense mutations present in two samples and approximately 89% of common missense mutations present in three samples were trunk mutations. Notably, trunk mutations appeared to have higher variant allele frequencies (VAFs) than non-trunk mutations.

CONCLUSIONS:

Our results indicate that small-scale multiregional sampling and subsequent screening of low VAF somatic mutations might be a cost-effective strategy for identifying the majority of trunk mutations in gastric carcinoma.

• Authors: Zhan Zhou, Shanshan Wu, Jun Lai, Yuan Shi, Chixiao Qiu, Zhe Chen, Yufeng Wang, Xun Gu, Jie Zhou, Shuqing Chen
• Journal: BMC medical genomics (BMC Med Genomics) Vol. 10 Issue 1 Pg. 49 (07 17 2017) ISSN: 1755-8794 [Electronic] England
• PMID: 28716121 (Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Adenocarcinoma (genetics, pathology)
• Carcinogenesis
• Clonal Evolution
• DNA Mutational Analysis (methods)
• Exome
• Genetic Heterogeneity
• Humans
• Male
• Middle Aged
• Mutation, Missense
• Precision Medicine (methods)
• Stomach Neoplasms (genetics, pathology)