Abstract | Background: Methods:
Remifentanil (1 µg ċ kg−1 ċ min−1) was given intravenously over 60 min in rats, followed by the infusion of either vehicle solution or the respective inhibitors of protein kinase C ( chelerythrine), extracellular signal-regulated kinase II ( KN93), or extracellular signal-regulated kinase 1/2 ( PD98059). Thereafter, the pain behaviors were evaluated by the paw withdrawal mechanical threshold and paw withdrawal thermal latency. In in vitro studies, fetal spinal cord dorsal horn neurons were primary cultured in the presence of 4 nM remifentanil for 60 min, and then the remifentanil was washed out and replaced immediately by culturing in the absence or presence of chelerythrine, KN93 or PD98059, respectively for up to 8 h. The expressions of N-methyl-D-aspartate receptors subunits and their phosphorylation (NR1, NR2B, p-NR1, p-NR2B) were analyzed by Western blotting after the completion of treatments. Functional changes of N-methyl-D-aspartate receptors were evaluated by electrophysiologic recordings of N-methyl-D-aspartate currents. Results: Conclusions:
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Authors | Sisi Li, Jie Zeng, Xiaoxiao Wan, Ying Yao, Nan Zhao, Yujia Yu, Cong Yu, Zhengyuan Xia |
Journal | Molecular pain
(Mol Pain)
2017 Jan-Dec
Vol. 13
Pg. 1744806917723789
ISSN: 1744-8069 [Electronic] United States |
PMID | 28714352
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Analgesics, Opioid
- Piperidines
- Receptors, N-Methyl-D-Aspartate
- Calcium-Calmodulin-Dependent Protein Kinase Type 2
- Remifentanil
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Topics |
- Analgesics, Opioid
(metabolism, pharmacology)
- Animals
- Calcium-Calmodulin-Dependent Protein Kinase Type 2
(drug effects, metabolism)
- Hyperalgesia
(drug therapy, metabolism)
- Male
- Pain, Postoperative
(drug therapy)
- Phosphorylation
(drug effects)
- Piperidines
(pharmacology)
- Posterior Horn Cells
(drug effects, metabolism)
- Rats, Sprague-Dawley
- Receptors, N-Methyl-D-Aspartate
(drug effects, metabolism)
- Remifentanil
- Spinal Cord
(drug effects, metabolism)
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