Comparative proteomic analysis of differentially expressed proteins in the Bombyx mori fat body during the microsporidia Nosema bombycis infection.

Abstract

Nosema bombycis is an obligate intracellular parasite, which can cause pébrine disease. To investigate the effects of N. bombycis infection, 5th-instar silkworms were challenged with N. bombycis isolate CQ1, and two-dimensional gel electrophoresis analysis was performed to analyze the differentially expressed proteins in infected and uninfected silkworm fat bodies 1, 2, 4, 6 and 8days post-infection (dpi). 46 differentially expressed proteins were identified at the 5 time points using MALDI-TOF/TOF MS. The changed proteins mainly involved in immune response, energy metabolism, and molecular synthesis. Overall, the identified proteins may provide important insights into the mechanisms of the silkworm response to N. bombycis infection.

• Authors: ZhiHong Li, GuoQing Pan, ZhenGang Ma, Bing Han, Bin Sun, Qi Ni, Jie Chen, Tian Li, TongBao Liu, MengXian Long, ChunFeng Li, ZeYang Zhou
• Journal: Journal of invertebrate pathology (J Invertebr Pathol) Vol. 149 Pg. 36-43 (10 2017) ISSN: 1096-0805 [Electronic] United States
• PMID: 28668257 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
• Copyright: Copyright © 2017. Published by Elsevier Inc.

Topics

• Animals
• Bombyx (metabolism, microbiology)
• Fat Body (metabolism)
• Microsporidiosis (metabolism)
• Nosema (physiology)
• Proteomics