Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: STUDY AIM: To study the anti-angiogenic properties of the water extract of EP vinegar preparation (WEVEP). MATERIALS AND METHODS: Following WEVEP treatment, intersegmental blood vessels were assessed during the development of transgenic Tg (flk: mCherry) zebrafish as was the proliferation, migration and network formation of HUVECs in vitro. mRNA expression of specific angiogenic-related genes including VEGF family members, Met, and NRP2 was also measured using quantitative real-time PCR (Q-PCR). RESULTS: Data demonstrated that angiogenesis was inhibited by the WEVEP in zebrafish (from 100µg/mL to 250µg/mL, p < 0.0001) and in the HUVEC model (from 100µg/mL to 400µg/mL, p < 0.0001). In the zebrafish model, the mean vessel numbers of administered groups were 26.00 ± 1.29 (100µg/mL), 24.54 ± 2.20 (150µg/mL), 22.66 ± 2.68 (200µg/mL), 20.80 ± 1.75 (250µg/mL), compared to 27.67 ± 0.96 of control group. Relative quantitative gene expression in zebrafish treated with WEVEP demonstrated that only VEGFR3 was significantly increased and other 23 genes including Met, VEGFA, Flt-1 were significantly decreased. CONCLUSION: WEVEP can positively modulate angiogenesis via multiple targeting mechanisms. Our novel results contribute towards the discovery of a possible mechanism(s) of the traditional use of EP in the treatment of cancer and malignant ascites.
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Authors | Wenting Zhang, Bin Liu, Yaru Feng, Jie Liu, Zhiqiang Ma, Jian Zheng, Qing Xia, Yuanyuan Ni, Farong Li, Ruichao Lin |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 206
Pg. 337-346
(Jul 12 2017)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 28602865
(Publication Type: Journal Article)
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Copyright | Copyright © 2017. Published by Elsevier B.V. |
Chemical References |
- Plant Extracts
- RNA, Messenger
- Water
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Topics |
- Animals
- Animals, Genetically Modified
- Cell Movement
(drug effects)
- Cell Proliferation
(drug effects)
- Euphorbia
(chemistry)
- Human Umbilical Vein Endothelial Cells
- Humans
- Neovascularization, Pathologic
(drug therapy, prevention & control)
- Plant Extracts
(pharmacology, therapeutic use)
- RNA, Messenger
(genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Water
- Zebrafish
(embryology)
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