Evaluation of HLA-G 14-bp ins/del and +3142G>C polymorphisms with susceptibility to recurrent spontaneous abortion.

Abstract

OBJECTIVE:

HLA-G is critically important for successful implantation during pregnancy. Increasing evidence supposed that HLA-G plays a key role in tolerance of the semi-allogeneic graft in pregnancy by inhibiting the cytotoxic functions of T and NK cells. The present study aimed to evaluate the impact of HLA-G rs1063320 (+3142G>C) and 14-bp insertion (ins)/deletion (del) polymorphisms on recurrent spontaneous abortion (RSA).

MATERIALS AND METHODS:

Genomic DNA from 93 RSA patients and 93 normal fertile women was isolated using the salting out method. Genotyping of HLA-G +3142G>C and 14-bp ins/del variants was done by polymerase chain reaction restriction fragment length polymorphism (PCR-RFP) and PCR method, respectively.

RESULTS:

The HLA-G +3142G>C polymorphism increased the risk of RSA in codominant (OR = 2.39, 95%CI = 1.27-4.49, p = 0.010, GC vs GG; OR = 3.28, 95%CI = 1.16-9.72, p = 0.040, CC vs GG) and dominant (OR = 2.52, 95%CI = 1.37-4.64, p = 0.004, GC + CC vs GG) tested inheritance models. HLA-G rs1063320 C allele was associated with increased risk of RSA (OR = 1.84, 95%CI = 1.20-2.83, p = 0.007). The del/del genotype as well as del allele of 14-bp ins/del variant increased that risk of RSA (OR = 3.02, 95%CI = 1.23-7.41, p = 0.025 and OR = 1.65, 95%CI = 1.09-2.50, p = 0.022, respectively).

CONCLUSION:

In summary, our results showed that HLA-G gene polymorphisms significantly increased the risk of RSA in a sample of the Iranian population.

• Authors: Mohammad Hashemi, Mojgan Mokhtari, Safura Khazaeian, Gholamreza Bahari, Maryam Rezaei, Alireza Nakhaee, Mohsen Taheri
• Journal: Taiwanese journal of obstetrics & gynecology (Taiwan J Obstet Gynecol) Vol. 56 Issue 3 Pg. 276-280 (Jun 2017) ISSN: 1875-6263 [Electronic] China (Republic : 1949- )
• PMID: 28600033 (Publication Type: Evaluation Study, Journal Article)
• Copyright: Copyright © 2017. Published by Elsevier B.V.

Chemical References

• HLA-G Antigens

Topics

• Abortion, Habitual (genetics, immunology)
• Alleles
• Case-Control Studies
• Female
• Gene Frequency
• HLA-G Antigens (genetics, immunology)
• Humans
• Iran
• Polymorphism, Genetic
• Pregnancy
• Real-Time Polymerase Chain Reaction
• Risk Factors