Microscopic Analysis and Quality Assessment of Induced Sputum From Children With Pneumonia in the PERCH Study.

Abstract

BACKGROUND.:

It is standard practice for laboratories to assess the cellular quality of expectorated sputum specimens to check that they originated from the lower respiratory tract. The presence of low numbers of squamous epithelial cells (SECs) and high numbers of polymorphonuclear (PMN) cells are regarded as indicative of a lower respiratory tract specimen. However, these quality ratings have never been evaluated for induced sputum specimens from children with suspected pneumonia.

METHODS.:

We evaluated induced sputum Gram stain smears and cultures from hospitalized children aged 1-59 months enrolled in a large study of community-acquired pneumonia. We hypothesized that a specimen representative of the lower respiratory tract will contain smaller quantities of oropharyngeal flora and be more likely to have a predominance of potential pathogens compared to a specimen containing mainly saliva. The prevalence of potential pathogens cultured from induced sputum specimens and quantity of oropharyngeal flora were compared for different quantities of SECs and PMNs.

RESULTS.:

Of 3772 induced sputum specimens, 2608 (69%) had <10 SECs per low-power field (LPF) and 2350 (62%) had >25 PMNs per LPF, measures traditionally associated with specimens from the lower respiratory tract in adults. Using isolation of low quantities of oropharyngeal flora and higher prevalence of potential pathogens as markers of higher quality, <10 SECs per LPF (but not >25 PMNs per LPF) was the microscopic variable most associated with high quality of induced sputum.

CONCLUSIONS.:

Quantity of SECs may be a useful quality measure of induced sputum from young children with pneumonia.

• Authors: David R Murdoch, Susan C Morpeth, Laura L Hammitt, Amanda J Driscoll, Nora L Watson, Henry C Baggett, W Abdullah Brooks, Maria Deloria Knoll, Daniel R Feikin, Karen L Kotloff, Orin S Levine, Shabir A Madhi, Katherine L O'Brien, J Anthony G Scott, Donald M Thea, Dilruba Ahmed, Juliet O Awori, Andrea N DeLuca, Bernard E Ebruke, Melissa M Higdon, Possawat Jorakate, Ruth A Karron, Sidi Kazungu, Geoffrey Kwenda, Lokman Hossain, Sirirat Makprasert, David P Moore, Azwifarwi Mudau, John Mwaba, Sandra Panchalingam, Daniel E Park, Christine Prosperi, Rasheed Salaudeen, Aliou Toure, Scott L Zeger, Stephen R C Howie, PERCH Study Group
• Journal: Clinical infectious diseases : an official publication of the Infectious Diseases Society of America (Clin Infect Dis) Vol. 64 Issue suppl_3 Pg. S271-S279 (Jun 15 2017) ISSN: 1537-6591 [Electronic] United States
• PMID: 28575360 (Publication Type: Journal Article, Multicenter Study)
• Copyright: © The Author 2017. Published by Oxford University Press for the Infectious Diseases Society of America.

Topics

• Bacteria (isolation & purification, ultrastructure)
• Child Health
• Child, Preschool
• Community-Acquired Infections (diagnosis, etiology)
• Epithelial Cells (ultrastructure)
• Female
• Hospitalization
• Humans
• Infant
• Infant, Newborn
• Male
• Neutrophils (ultrastructure)
• Pneumonia (diagnosis, etiology, microbiology)
• Pneumonia, Bacterial (diagnosis, microbiology)
• Respiratory Tract Infections (diagnosis, microbiology)
• Saliva (cytology, microbiology)
• Specimen Handling
• Sputum (cytology, microbiology)