Anti-inflammatory potential of hentriacontane in LPS stimulated RAW 264.7 cells and mice model.

Abstract

Hentriacontane, has various pharmacological effects including anti-inflammatory, antitumor and antimicrobial activities. Its anti-inflammatory potential has been demonstrated in peritoneal macrophages. However detailed studies on other models elucidating the mechanistic description of the mode of action has not been done. Hence, the aim of the present study is to evaluate the anti-inflammatory potential of hentriacontane both in-vivo (Balb/c mice) and in-vitro (RAW 264.7 cells). Cytokine inhibition of both pro-inflammatory (TNF-α, IL-6, MCP-1 and IL-1β) and anti-inflammatory (IL-10) cytokines was studied in RAW 264.7 cells and Balb/c mice. Suppressive potential of hentriacontane on NO, PGE2, LTB4 and on LPS induced translocation of NF-κB in RAW 264.7 cells was studied. Further investigations on the effect of hentriacontane on phagocytic index, carrageenan induced paw oedema in mice and on organ weight were done. It was found that hentriacontane significantly reduced all the parameters of inflammation in the experiments under study at all the concentrations, 10μM, 5μM and 1μM (in-vitro) and 5mg/kg, 2mg/kg and 1mg/kg (in-vivo). The highest concentration used in the two models presented the most significant results. The results indicate that hentriacontane is a potent suppressor of inflammatory cytokines and other mediators. Moreover it also has regulatory effect on NF-κB. Hence, hentriacontane is a potential candidate for investigations to develop anti-inflammatory drug.

• Authors: Vidushi Khajuria, Shilpa Gupta, Neha Sharma, Ashok Kumar, Nazir A Lone, Mowkshi Khullar, Prabhu Dutt, Parduman Raj Sharma, Asha Bhagat, Zabeer Ahmed
• Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 92 Pg. 175-186 (Aug 2017) ISSN: 1950-6007 [Electronic] France
• PMID: 28549290 (Publication Type: Journal Article)
• Copyright: Copyright © 2017 Elsevier Masson SAS. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Cytokines
• Hydrocarbons
• Inflammation Mediators
• Lipopolysaccharides
• NF-kappa B
• hentriacontane

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Cell Survival (drug effects, physiology)
• Cytokines (antagonists & inhibitors, metabolism)
• Dose-Response Relationship, Drug
• Female
• Hydrocarbons (pharmacology)
• Inflammation Mediators (antagonists & inhibitors, metabolism)
• Lipopolysaccharides (toxicity)
• Macrophages (drug effects, metabolism, pathology)
• Mice
• Mice, Inbred BALB C
• NF-kappa B (antagonists & inhibitors, metabolism)
• RAW 264.7 Cells