Recombinant vaccinia vector-based vaccine (Tiantan) boosting a novel HBV subunit vaccine induced more robust and lasting immunity in rhesus macaques.

Abstract

This study explored several prime-boost strategies in rhesus macaques using various novel hepatitis B virus (HBV) vaccines that showed promise as prophylactic and therapeutic approaches in our previous study using in a mouse model. The tested vaccines included an HBV particle subunit (HBSS1) vaccine and the recombinant vaccinia (RVJSS1) or adenoviral (rAdSS1) vector-based vaccines containing S (1-223aa) and PreS1 (21-47aa). The strength and maintenance of humoral activity (IgG and neutralizing antibodies) and cellular immunity (interferon-γ production assessed by IFN-γ enzyme-linked immunosorbent spot (ELISpot) assay) were investigated in a longitudinal study following various vaccination protocols until 79weeks post-vaccination. We found that HBSS1/RVJSS1 heterologous prime-boost elicits similar strong humoral immunity but more robust and lasting cellular immunity (CMI) than HBSS1/HBSS1 homologous vaccination in rhesus macaques. Furthermore, HBSS1/RVJSS1/RVJSS1 induced more robust and lasting CMI in macaques than did HBSS1/HBSS1/rAdSS1 vaccination. Therefore, HBSS1/RVJSS1/RVJSS1 is most promising candidates for protecting humans against HBV infection, especially for therapeutic application.

• Authors: Yao Deng, Xia Chuai, Pan Chen, Hong Chen, Wen Wang, Li Ruan, Wenhui Li, Wenjie Tan
• Journal: Vaccine (Vaccine) Vol. 35 Issue 25 Pg. 3347-3353 (06 05 2017) ISSN: 1873-2518 [Electronic] Netherlands
• PMID: 28487055 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2017 Elsevier Ltd. All rights reserved.

Chemical References

• Antibodies, Neutralizing
• Hepatitis B Antibodies
• Hepatitis B Vaccines
• Vaccines, Subunit
• Vaccines, Synthetic
• Interferon-gamma

Topics

• Animals
• Antibodies, Neutralizing (blood)
• Female
• Genetic Vectors
• Hepatitis B (prevention & control, therapy)
• Hepatitis B Antibodies (blood)
• Hepatitis B Vaccines (administration & dosage, genetics, immunology, therapeutic use)
• Immunity, Cellular
• Immunity, Humoral
• Immunization Schedule
• Immunization, Secondary
• Interferon-gamma (biosynthesis, immunology)
• Longitudinal Studies
• Macaca mulatta
• Male
• Mice
• Vaccination
• Vaccines, Subunit (immunology)
• Vaccines, Synthetic (administration & dosage, immunology)
• Vaccinia (genetics)