Evaluation of recombinant adenovirus vaccines based on glycoprotein D and truncated UL25 against herpes simplex virus type 2 in mice.

Abstract

The high prevalence of herpes simplex virus 2 (HSV-2) infections in humans necessitates the development of a safe and effective vaccine that will need to induce vigorous T-cell responses to control viral infection and transmission. We designed rAd-gD2, rAd-gD2ΔUL25, and rAd-ΔUL25 to investigate whether recombinant replication-defective adenoviruses vaccine could induce specific T-cell responses and protect mice against intravaginal HSV-2 challenge compared with FI-HSV-2. In the present study, recombinant adenovirus-based HSV-2 showed higher reductions in mortality and stronger antigen-specific T-cell responses compared with FI-HSV-2 and the severity of genital lesions in mice immunized with rAd-gD2ΔUL25 was significantly decreased by eliciting IFN-γ-secreting T-cell responses compared with rAd-gD2 and rAd-ΔUL25 groups. Our results demonstrated the immunogenicity and protective efficacy of recombinant adenovirus vaccines in acute HSV-2 infection following intravaginal challenge in mice.

• Authors: Wei Liu, Yan Zhou, Ziyan Wang, Zeqiang Zhang, Qizhi Wang, Weiheng Su, Yan Chen, Yan Zhang, Feng Gao, Chunlai Jiang, Wei Kong
• Journal: Microbiology and immunology (Microbiol Immunol) Vol. 61 Issue 5 Pg. 176-184 (May 2017) ISSN: 1348-0421 [Electronic] Australia
• PMID: 28378925 (Publication Type: Journal Article)
• Copyright: © 2017 The Societies and John Wiley & Sons Australia, Ltd.

Chemical References

• Adenovirus Vaccines
• Capsid Proteins
• Glycoproteins
• IFNG protein, mouse
• UL25 protein, herpes simplex virus type 2
• Vaccines, Synthetic
• Viral Envelope Proteins
• Interferon-gamma

Topics

• Adenoviridae (immunology)
• Adenovirus Vaccines (immunology)
• Administration, Intravaginal
• Animals
• Capsid Proteins (genetics, immunology)
• Disease Models, Animal
• Female
• Glycoproteins (immunology)
• Herpes Genitalis (immunology, prevention & control, virology)
• Herpesvirus 2, Human (immunology)
• Interferon-gamma (immunology)
• Mice
• Mice, Inbred BALB C
• Vaccines, Synthetic (immunology)
• Viral Envelope Proteins (immunology)