Abstract | OBJECTIVE: METHODS: A total of 45 Sprague Dawley rats were randomly divided into three groups: control, control diabetes mellitus and fluctuated blood glucose groups. Fluctuated blood glucose was induced by daily subcutaneous insulin and intraperitoneal glucose injections at different time points. Blood lipids and glycosylated haemoglobin A1c were assessed. Super oxide dismutase activity and malondialdehyde level in rat heart homogenates were determined by assay kit. Structural cardiac tissue changes were observed by haematoxylin and eosin staining and Masson's trichrome staining. Collagen type 3, fibronectin, phosphorylated Ser/Thr protein kinase, phosphorylated glycogen synthase kinase-3 beta, glycogen synthase kinase-3 beta, nuclear factor kappa-light-chain-enhancer of activated B cells, cleaved-cysteinyl aspartate-specific proteinase-3 (caspase-3) and tumour necrosis factor-α levels were determined by western blot. RESULTS: CONCLUSION:
Blood glucose variability can aggravate heart tissue fibrosis, possibly involving oxidative stress by inhibiting AKT signalling path.
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Authors | Changjiang Ying, Ting Liu, Hongwei Ling, Mingyue Cheng, Xiaoyan Zhou, Shanshan Wang, Yizhen Mao, Lei Chen, Runze Zhang, Wei Li |
Journal | Diabetes & vascular disease research
(Diab Vasc Dis Res)
Vol. 14
Issue 4
Pg. 327-335
(07 2017)
ISSN: 1752-8984 [Electronic] England |
PMID | 28301953
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- Blood Glucose
- Glycated Hemoglobin A
- Lipids
- NF-kappa B
- Tumor Necrosis Factor-alpha
- Glycogen Synthase Kinase 3 beta
- Gsk3b protein, rat
- Proto-Oncogene Proteins c-akt
- Casp3 protein, rat
- Caspase 3
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Topics |
- Animals
- Biomarkers
(blood)
- Blood Glucose
(metabolism)
- Caspase 3
(metabolism)
- Diabetes Mellitus, Experimental
(blood, complications)
- Diabetes Mellitus, Type 2
(blood, complications)
- Diabetic Cardiomyopathies
(blood, enzymology, etiology, pathology)
- Fibrosis
- Glycated Hemoglobin
(metabolism)
- Glycogen Synthase Kinase 3 beta
(metabolism)
- Lipids
(blood)
- Male
- Myocardium
(enzymology, pathology)
- NF-kappa B
(metabolism)
- Oxidative Stress
- Proto-Oncogene Proteins c-akt
(metabolism)
- Rats, Sprague-Dawley
- Signal Transduction
- Time Factors
- Tumor Necrosis Factor-alpha
(metabolism)
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