Abstract |
Acute lung injury (ALI) is one of the most important complications after cardiopulmonary bypass (CPB) and the complex pathophysiology remains to be resolved incomplete. SDF-1/CXCR4 chemokine axis can chemotactically accumulate inflammatory cell to local tissue and regulate the release of inflammatory factors, and SDF-1 has a strong chemotaxis effect on neutrophils with CXCR4. Since CPB animal model was difficult to establish, there was still no report about the effect of SDF-1/CXCR4 on neutrophil chemotaxis in ALI after CPB. Here, a stable CPB rat model was constructed to clarify the role of SDF-1/CXCR4 axis in the CPB-induced ALI. Real-time quantitative PCR (RT-qPCR), Western blot analysis, and enzyme-linked immunosorbent assay (ELISA) were used to detect the changes of SDF-1 and CXCR4 in lung tissues, blood, bronchoalveolar lavage (BALF), and/or isolated neutrophils. SDF-1/CXCR4 was increased after CPB, both of that were increased in blood; CXCR4 was increased in neutrophils; SDF-1/CXCR4 was also increased in BALF of CPB model. Results indicated that SDF-1/CXCR4 axis played a key role in the process of early ALI after CPB, also showed that lung injury was significantly reduce after blocking SDF-1/CXCR4 axis, suggest that CXCR4 might be a new target for ALI treatment.
|
Authors | Hai Shi, Rujian Lu, Shuo Wang, Honglin Chen, Fei Wang, Kun Liu |
Journal | Inflammation
(Inflammation)
Vol. 40
Issue 3
Pg. 937-945
(Jun 2017)
ISSN: 1573-2576 [Electronic] United States |
PMID | 28285461
(Publication Type: Journal Article)
|
Chemical References |
- CXCL12 protein, rat
- Chemokine CXCL12
- Cxcr4 protein, rat
- Receptors, CXCR4
|
Topics |
- Acute Lung Injury
(etiology)
- Animals
- Cardiopulmonary Bypass
(adverse effects)
- Chemokine CXCL12
(analysis, pharmacology)
- Chemotaxis
- Male
- Neutrophils
(chemistry, drug effects)
- Rats
- Rats, Sprague-Dawley
- Receptors, CXCR4
(analysis, physiology)
|