Collagen-based silver nanoparticles: Study on cell viability, skin permeation, and swelling inhibition.

Abstract

Collagen is considered the most abundant protein in the animal kingdom, comprising 30% of the total amount of proteins and 6% of the human body by weight. Studies that examine the interaction between silver nanoparticles and proteins have been highlighted in the literature in order to understand the stability of the nanoparticle system, the effects observed in biological systems, and the appearance of new chemical pharmaceutical products. The objective of this study was to analyze the behavior of silver nanoparticles stabilized with collagen (AgNPcol) and to check the skin permeation capacity and action in paw edema induced by carrageenan. AgNPcol synthesis was carried out using solutions of reducing agent sodium borohydride (NaBH4), silver nitrate (AgNO3) and collagen. Characterization was done by using dynamic light scattering (DLS) and X-ray diffraction (XRD) and AFM. Cellular viability testing was performed by using flow cytometry in human melanoma cancer (MV3) and murine fibroblast (L929) cells. The skin permeation study was conducted using a Franz diffusion cell, and the efficiency of AgNPcol against the formation of paw edema in mice was evaluated. The hydrodynamic diameter and zeta potential of AgNPcol were 140.7±7.8nm and 20.1±0.7mV, respectively. AgNPcol failed to induce early apoptosis, late apoptosis, and necrosis in L929 cells; however, it exhibited enhanced toxicity in cancer cells (MV3) compared to normal cells (L929). AgNPcol demonstrated increased toxicological effects in cancer MV3 cells, promoting skin permeation, and preventing paw edema.

• Authors: Vinicius Saura Cardoso, Marcelo de Carvalho Filgueiras, Yago Medeiros Dutra, Ramon Handerson Gomes Teles, Alyne Rodrigues de Araújo, Fernando Lucas Primo, Ana Carolina Mafud, Larissa Fernandes Batista, Yvonne Primerano Mascarenhas, Iêda Maria Martinez Paino, Valtencir Zucolotto, Antonio Claudio Tedesco, Durcilene Alves Silva, José Roberto S A Leite, José Ribeiro Dos Santos Jr
• Journal: Materials science & engineering. C, Materials for biological applications (Mater Sci Eng C Mater Biol Appl) Vol. 74 Pg. 382-388 (May 01 2017) ISSN: 1873-0191 [Electronic] Netherlands
• PMID: 28254308 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.

Chemical References

• Biocompatible Materials
• Borohydrides
• Reactive Oxygen Species
• Silver
• sodium borohydride
• Carrageenan
• Collagen
• Silver Nitrate

Topics

• Animals
• Apoptosis (drug effects)
• Biocompatible Materials (chemistry, pharmacology, therapeutic use)
• Borohydrides (chemistry)
• Carrageenan (toxicity)
• Cell Line
• Collagen (chemistry)
• Dynamic Light Scattering
• Edema (chemically induced, drug therapy)
• Humans
• Male
• Metal Nanoparticles (chemistry, toxicity)
• Mice
• Microscopy, Atomic Force
• Permeability (drug effects)
• Reactive Oxygen Species (metabolism)
• Silver (chemistry)
• Silver Nitrate (chemistry)
• Skin (drug effects, metabolism)
• X-Ray Diffraction