Role of Periostin in Early Brain Injury After Subarachnoid Hemorrhage in Mice.

Abstract

BACKGROUND AND PURPOSE:

A matricellular protein tenascin-C is implicated in early brain injury after experimental subarachnoid hemorrhage (SAH). This study first evaluated the role of another matricellular protein periostin and the relationships with tenascin-C in post-SAH early brain injury.

METHODS:

Wild-type (n=226) and tenascin-C knockout (n=9) C57BL/6 male adult mice underwent sham or filament perforation SAH modeling. Vehicle, anti-periostin antibody, or recombinant periostin was randomly administrated by an intracerebroventricular injection at 30 minutes post-modeling. Neuroscores, SAH grading, brain water content, immunostaining, and Western blotting were blindly evaluated at 24 to 48 hours post-SAH.

RESULTS:

Periostin was induced in brain capillary endothelial cells and neurons at 24 hours post-SAH. Anti-periostin antibody improved post-SAH neurobehavior, brain edema, and blood-brain barrier disruption associated with downregulation of tenascin-C, inactivation of p38, extracellular signal-related kinase 1/2 and matrix metalloproteinase-9, and subsequent preservation of zona occludens-1. Recombinant periostin aggravated post-SAH brain edema and tenascin-C induction. Tenascin-C knockout prevented post-SAH neurobehavioral impairments and periostin induction.

CONCLUSIONS:

Periostin may cause post-SAH early brain injury through activating downstream signaling pathways and interacting with tenascin-C, providing a novel approach for the treatment of early brain injury.

• Authors: Lei Liu, Fumihiro Kawakita, Masashi Fujimoto, Fumi Nakano, Kyoko Imanaka-Yoshida, Toshimichi Yoshida, Hidenori Suzuki
• Journal: Stroke (Stroke) Vol. 48 Issue 4 Pg. 1108-1111 (04 2017) ISSN: 1524-4628 [Electronic] United States
• PMID: 28242775 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2017 American Heart Association, Inc.

Chemical References

• Antibodies
• Cell Adhesion Molecules
• Postn protein, mouse
• Recombinant Proteins
• Tenascin

Topics

• Animals
• Antibodies
• Blood-Brain Barrier (metabolism)
• Brain Injuries (metabolism)
• Cell Adhesion Molecules (immunology, metabolism)
• Disease Models, Animal
• Male
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Recombinant Proteins
• Subarachnoid Hemorrhage (complications, metabolism)
• Tenascin (metabolism)