Bone marrow mesenchymal stem cells-conditioned medium enhances vascular remodeling after stroke in type 2 diabetic rats.

Abstract

Our previous studies have found that stem cells conditioned medium (CM) facilitated functional recovery after stroke in non-diabetics. However, whether bone marrow stromal cells conditioned medium (BMSCs-CM) treatment after stroke in type 2 diabetic (T2DM) rats improve functional outcomes remains unclear. T2DM rats were induced and subjected to stroke then treated with or without BMSCs-CM. Functional outcomes and blood-brain barrier (BBB) leakage were performed, the expression of Angiopoietin (Ang) 1 and tyrosine kinase (Tie) 2 were also assessed. Our results showed that BMSCs-CM treatment significantly improved functional outcomes, decreased BBB leakage and the expression of Ang1 and Tie2 were also changed after BMSCs-CM treatment in type 2 diabetes after stroke. In conclusion, enhanced expression of Ang1 and Tie2 in ischemic brain after BMSCs-CM treatment of stroke may contribute to the improved functional recovery after stroke in type 2 diabetic rats.

• Authors: Jie Xiang, Jinxia Hu, Tong Shen, Bin Liu, Fang Hua, Kun Zan, Jie Zu, Guiyun Cui, Xinchun Ye
• Journal: Neuroscience letters (Neurosci Lett) Vol. 644 Pg. 62-66 (03 22 2017) ISSN: 1872-7972 [Electronic] Ireland
• PMID: 28219791 (Publication Type: Journal Article)
• Copyright: Copyright © 2017 Elsevier B.V. All rights reserved.

Chemical References

• Angiopoietin-1
• Culture Media, Conditioned
• Receptor, TIE-2

Topics

• Angiopoietin-1 (biosynthesis)
• Animals
• Bone Marrow Cells
• Brain (metabolism, pathology)
• Culture Media, Conditioned (pharmacology)
• Diabetes Mellitus, Experimental (complications, metabolism, pathology)
• Diabetes Mellitus, Type 2
• Male
• Mesenchymal Stem Cell Transplantation
• Mesenchymal Stem Cells (drug effects)
• Rats
• Rats, Wistar
• Receptor, TIE-2 (biosynthesis)
• Recovery of Function (drug effects)
• Stroke (complications, metabolism, pathology)
• Vascular Remodeling