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microRNA-625 inhibits tumorigenicity by suppressing proliferation, migration and invasion in malignant melanoma.

Abstract
Dysregulated microRNA (miR)-625 expression has been observed in several kinds of cancer. MicroRNAs are important factors in the development and progression of malignant melanoma, though the clinical significance and function of miR-625 in human malignant melanoma remain unclear. Levels of miR-625 expression were therefore determined in 36 pairs of malignant melanoma and adjacent non-tumor tissue using qPCR. The effects of miR-625 dysregulation on malignant melanoma cell proliferation, wound healing, migration and invasion in vitro and tumorigenicity in vivo were investigated using CCK-8, transwell assays, and a nude mouse subcutaneous tumor model. Bioinformatics analysis and luciferase reporter system were used to predict and confirm the target gene of miR-625. miR-625 levels were frequently decreased in malignant melanoma. Ectopic expression of miR-625 suppressed proliferation, wound healing, migration, and tumorgenicity in malignant melanoma. Moreover, miR-625 acted, at least in part, by suppressing potential target SOX2. These results show that miR-625 is a tumor suppressor that inhibits the development and progression of malignant melanoma, which suggests miR-625 is potentially a new diagnostic marker and therapeutic target of malignant melanoma.
AuthorsWei Fang, Yibin Fan, Zhenzong Fa, Jinhua Xu, Hongyu Yu, Pu Li, Julin Gu
JournalOncotarget (Oncotarget) Vol. 8 Issue 8 Pg. 13253-13263 (Feb 21 2017) ISSN: 1949-2553 [Electronic] United States
PMID28129648 (Publication Type: Journal Article)
Chemical References
  • 3' Untranslated Regions
  • MIRN625 microRNA, human
  • MicroRNAs
  • SOX2 protein, human
  • SOXB1 Transcription Factors
Topics
  • 3' Untranslated Regions (genetics)
  • Animals
  • Cell Line, Tumor
  • Cell Movement (genetics)
  • Cell Proliferation (genetics)
  • Gene Expression Regulation, Neoplastic
  • HEK293 Cells
  • Humans
  • Male
  • Melanoma (genetics, pathology)
  • Mice, Inbred BALB C
  • Mice, Nude
  • MicroRNAs (genetics)
  • Neoplasm Invasiveness
  • Reverse Transcriptase Polymerase Chain Reaction
  • SOXB1 Transcription Factors (genetics)
  • Transplantation, Heterologous
  • Tumor Burden (genetics)

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