Eradication of melanoma in vitro and in vivo via targeting with a Killer-Red-containing telomerase-dependent adenovirus.

Abstract

Melanoma is a highly recalcitrant cancer and transformative therapy is necessary for the cure of this disease. We recently developed a telomerase-dependent adenovirus containing the fluorescent protein Killer-Red. In the present report, we first determined the efficacy of Killer-Red adenovirus combined with laser irradiation on human melanoma cell lines in vitro. Cell viability of human melanoma cells was reduced in a dose-dependent and irradiation-time-dependent manner. We used an intradermal xenografted melanoma model in nude mice to determine efficacy of the Killer-Red adenovirus. Intratumoral injection of Killer-Red adenovirus, combined with laser irradiation, eradicated the melanoma indicating the potential of a new paradigm of cancer therapy.

• Authors: Kiyoto Takehara, Shuya Yano, Hiroshi Tazawa, Hiroyuki Kishimoto, Nobuhiro Narii, Hiroyuki Mizuguchi, Yasuo Urata, Shunsuke Kagawa, Toshiyoshi Fujiwara, Robert M Hoffman
• Journal: Cell cycle (Georgetown, Tex.) (Cell Cycle) Vol. 16 Issue 16 Pg. 1502-1508 (Aug 18 2017) ISSN: 1551-4005 [Electronic] United States
• PMID: 28055296 (Publication Type: Journal Article)

Chemical References

• killer red protein, Anthomedusae
• Green Fluorescent Proteins
• Telomerase

Topics

• Adenoviridae (metabolism)
• Animals
• Cell Line, Tumor
• Cell Survival (radiation effects)
• Green Fluorescent Proteins (metabolism)
• Humans
• Light
• Melanoma (pathology, radiotherapy)
• Mice, Nude
• Photochemotherapy
• Telomerase (metabolism)
• Time Factors