Modulation of intestinal barrier function to ameliorate Salmonella infection in mice by oral administration of fermented milks produced with Lactobacillus plantarum MTCC 5690 - a probiotic strain of Indian gut origin.

Abstract

Probiotic Lactobacillus plantarum MTCC 5690, a probiotic strain of Indian gut origin, and milk formulations produced with the same were explored in this study as biotherapeutics by evaluating their functional efficacy against Salmonella infection in mice. The efficacy of milk formulations (fermented/unfermented) of MTCC 5690 for enhancement of intestinal barrier function was determined by monitoring the permeability and histopathology of the intestine. Infected mice fed with probiotic Dahi, fermented probiotic drink and sweetened fermented probiotic drink maintained the health and integrity of the intestinal epithelium as compared to those fed with PBS, milk, unfermented probiotic milk and Dahi. Our relative expression data revealed that the changes caused by MTCC 5690 in intestinal barrier function components were established through modulation of the key regulatory receptors Toll-like receptor 2 and Toll-like receptor 4. The results suggest that fermented milks of MTCC 5690 could enhance the defences of the intestinal barrier in enteric infection condition and, therefore, can be explored as a dietary-based strategy to reduce Salmonella infection in the human gut.

• Authors: Namita Rokana, Rajbir Singh, Rashmi Hogarehalli Mallappa, Virender Kumar Batish, Sunita Grover
• Journal: Journal of medical microbiology (J Med Microbiol) Vol. 65 Issue 12 Pg. 1482-1493 (Dec 2016) ISSN: 1473-5644 [Electronic] England
• PMID: 27902414 (Publication Type: Journal Article)

Chemical References

• Muc2 protein, mouse
• Mucin-2
• Toll-Like Receptor 2
• Toll-Like Receptor 4

Topics

• Administration, Oral
• Animals
• Bacterial Translocation
• Cultured Milk Products (microbiology)
• Disease Models, Animal
• Feces (microbiology)
• India
• Intestinal Mucosa (microbiology, pathology, physiology)
• Intestines (microbiology, physiology)
• Irritable Bowel Syndrome (genetics)
• Lactobacillus plantarum (growth & development, isolation & purification, physiology)
• Mice
• Milk (microbiology)
• Mucin-2 (genetics)
• Polymerase Chain Reaction
• Probiotics (therapeutic use)
• Salmonella Infections, Animal (microbiology, therapy)
• Salmonella typhimurium (physiology)
• Toll-Like Receptor 2 (genetics)
• Toll-Like Receptor 4 (genetics)