Heat Increases the Editing Efficiency of Human Papillomavirus E2 Gene by Inducing Upregulation of APOBEC3A and 3G.

Abstract

Apolipoprotein B mRNA-editing catalytic polypeptide (APOBEC) 3 proteins have been identified as potent viral DNA mutators and have broad antiviral activity. In this study, we demonstrated that apolipoprotein B mRNA-editing catalytic polypeptide 3A (A3A) and A3G expression levels were significantly upregulated in human papillomavirus (HPV)-infected cell lines and tissues. Heat treatment resulted in elevated expression of A3A and A3G in a temperature-dependent manner in HPV-infected cells. Correspondingly, HPV-infected cells heat-treated at 44 °C showed accumulated G-to-A or C-to-T mutation in HPV E2 gene. Knockdown of A3A or A3G could promote cell viability, along with the lower frequency of A/T in HPV E2 gene. In addition, regressing genital viral warts also harbored high G-to-A or C-to-T mutation in HPV E2 gene. Taken together, we demonstrate that apolipoprotein B mRNA-editing catalytic polypeptide 3 expression and editing function was heat sensitive to a certain degree, partly explaining the mechanism of action of local hyperthermia to treat viral warts.

• Authors: Yang Yang, Hexiao Wang, Xinrui Zhang, Wei Huo, Ruiqun Qi, Yali Gao, Gaofeng Zhang, Bing Song, Hongduo Chen, Xinghua Gao
• Journal: The Journal of investigative dermatology (J Invest Dermatol) Vol. 137 Issue 4 Pg. 810-818 (04 2017) ISSN: 1523-1747 [Electronic] United States
• PMID: 27890786 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

Chemical References

• Proteins
• RNA, Messenger
• APOBEC3A protein, human
• Cytidine Deaminase

Topics

• Cells, Cultured
• Condylomata Acuminata (genetics, therapy, virology)
• Cytidine Deaminase (genetics)
• DNA Mutational Analysis
• Female
• Gene Expression Regulation
• Humans
• Hypothermia, Induced (methods)
• Male
• Papillomaviridae (genetics)
• Papillomavirus Infections (genetics, pathology, therapy)
• Proteins (genetics)
• RNA, Messenger (genetics)
• Sensitivity and Specificity
• Up-Regulation