Oral Helicobacter pylori vaccine-encapsulated acid-resistant HP55/PLGA nanoparticles promote immune protection.

Abstract

Oral vaccination, is notoriously weak or nonimmunogenic. One of the major reasons is the inefficient antigen uptake caused by enzymolysis and hydrolysis in the gastrointestinal tract. In this study, acid-resistant HP55/PLGA nanoparticle was developed as an oral delivery system to protect H. pylori recombinant antigen CCF against the complex gastrointestinal environment. These ∼200nm particles controlled the release of antigen in the acidic environment (pH⩽5.5). Immunized mice with HP55/PLGA-CCF nanoparticles induced high levels of urease-specific antibodies and memory T cell responses. A month after H. pylori challenge, 43% of mice were completely protected. The protection was highly associated with the Th1/Th17-bias immune response, which had been recognized as an optimal immunity against H. pylori infection. In addition, a mass of T-cells were observed in the lamina propria of mice immunized with CCF, especially in the HP55/PLGA-CCF nanoparticles administered recipients, and contributed to the development of postimmunization gastritis. These results indicate that oral immunization with acid-resistant HP55/PLGA nanoparticles encapsulating vaccine antigens represent a promising strategy for antigen protection, slow-release and targeting, and thus prevented gastrointestinal infection.

• Authors: Zhoulin Tan, Wei Liu, Hai Liu, Cheng Li, Yan Zhang, Xia Meng, Tingting Tang, Tao Xi, Yingying Xing
• Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V (Eur J Pharm Biopharm) Vol. 111 Pg. 33-43 (Feb 2017) ISSN: 1873-3441 [Electronic] Netherlands
• PMID: 27865932 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.

Chemical References

• Adjuvants, Immunologic
• Antibodies, Bacterial
• Antigens, Bacterial
• Bacterial Vaccines
• Immunoglobulin A
• Immunoglobulin G
• Polylactic Acid-Polyglycolic Acid Copolymer
• Polyglycolic Acid
• Lactic Acid

Topics

• Adjuvants, Immunologic
• Administration, Oral
• Animals
• Antibodies, Bacterial (immunology)
• Antigens, Bacterial (immunology)
• Bacterial Vaccines (administration & dosage)
• Gastric Mucosa (metabolism)
• Gastritis (immunology)
• Helicobacter Infections (prevention & control)
• Helicobacter pylori (immunology)
• Immunoglobulin A (immunology)
• Immunoglobulin G (immunology)
• Lactic Acid (chemistry)
• Lymphocytes (cytology)
• Male
• Mice
• Mice, Inbred BALB C
• Nanoparticles (chemistry)
• Polyglycolic Acid (chemistry)
• Polylactic Acid-Polyglycolic Acid Copolymer
• Real-Time Polymerase Chain Reaction