Abstract |
Recent evidence shows that miRNAs are dysregulated in a variety of cancers including gastric cancer (GC), and emerging as key oncogenes or tumor suppressors. In this study, qRT-PCR was used to analyze the expression of miR-186 in GC tissues and adjacent non-cancerous tissues, and then more in-vitro experiments were used to investigate the role of miR-186 in GC cells. Here, we identified miR-186 was generally down-regulated in GC tissues; however, Twist1 was generally up-regulated in GC tissues. Moreover, miR-186 and Twist1 were associated with larger tumor size and advanced clinical stage of GC. In-vitro experiments demonstrated that ectopic overexpression of miR-186 inhibited GC cell proliferation, invasion and migration; however, inhibited expression of miR-186 enhanced cell proliferation, invasion and migration. Furthermore, the luciferase reporter assay demonstrated Twist1 as a direct target of miR-186. Finally, over-expression of Twist1 abrogated inhibitory impact of miR-186 on cell proliferation, invasion and migration. In conclusion, miR-186 affects the proliferation, invasion and migration of human gastric cancer by inhibition of Twist1, and could be a tumor suppressor in GC development. Thus, miR-186 may be served as a candidate prognostic biomarker and target for new therapies in human gastric cancer.
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Authors | Chunhong Cao, Deguang Sun, Liang Zhang, Lei Song |
Journal | Oncotarget
(Oncotarget)
Vol. 7
Issue 48
Pg. 79956-79963
(Nov 29 2016)
ISSN: 1949-2553 [Electronic] United States |
PMID | 27835599
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- MIRN186 microRNA, human
- MicroRNAs
- Nuclear Proteins
- TWIST1 protein, human
- Twist-Related Protein 1
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Biomarkers, Tumor
(genetics)
- Cell Line, Tumor
- Cell Movement
(genetics)
- Cell Proliferation
(genetics)
- Female
- Gene Expression Regulation, Neoplastic
- Humans
- Male
- MicroRNAs
(physiology)
- Middle Aged
- Neoplasm Invasiveness
- Nuclear Proteins
(genetics)
- Stomach Neoplasms
(diagnosis, genetics, pathology)
- Twist-Related Protein 1
(genetics)
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