A distinct microbiota composition is associated with protection from food allergy in an oral mouse immunization model.

Abstract

In our mouse model, gastric acid-suppression is associated with antigen-specific IgE and anaphylaxis development. We repeatedly observed non-responder animals protected from food allergy. Here, we aimed to analyse reasons for this protection. Ten out of 64 mice, subjected to oral ovalbumin (OVA) immunizations under gastric acid-suppression, were non-responders without OVA-specific IgE or IgG1 elevation, indicating protection from allergy. In these non-responders, allergen challenges confirmed reduced antigen uptake and lack of anaphylactic symptoms, while in allergic mice high levels of mouse mast-cell protease-1 and a body temperature reduction, indicative for anaphylaxis, were determined. Upon OVA stimulation, significantly lower IL-4, IL-5, IL-10 and IL-13 levels were detected in non-responders, while IL-22 was significantly higher. Comparison of fecal microbiota revealed differences of bacterial communities on single bacterial Operational-Taxonomic-Unit level between the groups, indicating protection from food allergy being associated with a distinct microbiota composition in a non-responding phenotype in this mouse model.

• Authors: Susanne C Diesner, Cornelia Bergmayr, Barbara Pfitzner, Vera Assmann, Durga Krishnamurthy, Philipp Starkl, David Endesfelder, Michael Rothballer, Gerhard Welzl, Thomas Rattei, Thomas Eiwegger, Zsolt Szépfalusi, Heinz Fehrenbach, Erika Jensen-Jarolim, Anton Hartmann, Isabella Pali-Schöll, Eva Untersmayr
• Journal: Clinical immunology (Orlando, Fla.) (Clin Immunol) Vol. 173 Pg. 10-18 (Dec 2016) ISSN: 1521-7035 [Electronic] United States
• PMID: 27789346 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

Chemical References

• Allergens
• Anti-Ulcer Agents
• Cytokines
• Immunoglobulin A
• Immunoglobulin G
• Immunoglobulin E
• Sucralfate
• Ovalbumin

Topics

• Administration, Oral
• Allergens (administration & dosage)
• Anaphylaxis (immunology, microbiology)
• Animals
• Anti-Ulcer Agents (pharmacology)
• Bacteria (isolation & purification)
• Cytokines (immunology)
• Disease Models, Animal
• Feces (microbiology)
• Female
• Food Hypersensitivity (immunology, microbiology)
• Gastric Acid
• Immunization
• Immunoglobulin A (immunology)
• Immunoglobulin E (immunology)
• Immunoglobulin G (immunology)
• Intestines (anatomy & histology, immunology)
• Mice, Inbred BALB C
• Microbiota
• Ovalbumin (administration & dosage, blood)
• Spleen (cytology, immunology)
• Stomach (anatomy & histology, immunology)
• Sucralfate (pharmacology)