Evaluation of the cytotoxic, apoptosis inducing activity and molecular docking of spiroquinazolinone benzamide derivatives in MCF-7 breast cancer cells.

Abstract

Previous studies have suggested that quinazolinone derivatives are potent apoptosis-inducing agents in various cancer cell lines. In the present study, we have investigated cytotoxic, apoptosis induction, and molecular docking activities of the spiroquinazolinone benzamide derivatives family on MCF-7 human breast cancer cells. The MTT cytotoxicity assays and docking studies showed that 4t-CHQB was the most active compound among the prepared spiroquinazolinone benzamide compounds with IC50 of 50 ± 1.2 μM and was selected for further assessments. Apoptosis, as the mechanism of cell death, was assessed morphologically by acridine orange/ethidium bromide (AO/EtBr) double staining, evaluation of the cell surface phosphatidylserine (PS) expression through annexin V/PI technique and, the formation of DNA ladder. Down regulation of survivin was evaluated in protein level after cell treatment with 4t-CHQB using western blotting method. Molecular modeling experiments involving 4t-CHQB binding site of survivin showed several strong hydrogen bonds and hydrophobic interactions between many important amino acid residues. Overall, the obtained data suggest that the assessed spiroquinazolinone benzamide compounds may provide a novel therapeutic approach for further evaluation, as an effective chemotherapeutic family acting through down regulation of survivin and apoptosis induction in breast cancer.

• Authors: Majid Mahdavi, Malihe Mohseni Lavi, Reza Yekta, Mohammad Amin Moosavi, Mahnaz Nobarani, Saeed Balalaei, Sanam Arami, Mohammad Reza Rashidi
• Journal: Chemico-biological interactions (Chem Biol Interact) Vol. 260 Pg. 232-242 (Dec 25 2016) ISSN: 1872-7786 [Electronic] Ireland
• PMID: 27712999 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• BIRC5 protein, human
• Benzamides
• Inhibitor of Apoptosis Proteins
• Ligands
• Spiro Compounds
• Survivin
• spiroquinazoline
• benzamide

Topics

• Apoptosis (drug effects)
• Benzamides (chemistry, pharmacology)
• Breast Neoplasms (metabolism, pathology)
• Cell Cycle (drug effects)
• Cell Shape (drug effects)
• Cell Survival (drug effects)
• DNA Fragmentation (drug effects)
• Female
• Flow Cytometry
• Humans
• Inhibitor of Apoptosis Proteins (metabolism)
• Inhibitory Concentration 50
• Ligands
• MCF-7 Cells
• Molecular Docking Simulation
• Spiro Compounds (chemistry, pharmacology)
• Survivin
• Thermodynamics