In Vitro Effects of Some Botanicals with Anti-Inflammatory and Antitoxic Activity.

Abstract

Several extrinsic factors, like drugs and chemicals, can foster autoimmunity. Tetracyclines, in particular oxytetracycline (OTC), appear to correlate with the emergence of immune-mediated diseases. Accumulation of OTC, the elective drug for gastrointestinal and respiratory infectious disease treatment in broiler chickens, was reported in chicken edible tissues and could represent a potential risk for pets and humans that could assume this antibiotic as residue in meat or in meat-derived byproducts. We investigated the in vitro anti-inflammatory properties of a pool of thirteen botanicals as a part of a nutraceutical diet, with proven immunomodulatory activity. In addition, we evaluated the effect of such botanicals in contrasting the in vitro proinflammatory toxicity of OTC. Our results showed a significant reduction in interferon- (INF-) γ production by human and canine lymphocytes in presence of botanicals ((⁎) p < 0.05). Increased INF-γ production, dependent on 24-hour OTC-incubation of T lymphocytes, was significantly reduced by the coincubation with Haematococcus pluvialis, with Glycine max, and with the mix of all botanicals ((⁎) p < 0.05). In conclusion, the use of these botanicals was shown to be able to contrast OTC-toxicity and could represent a new approach for the development of functional foods useful to enhance the standard pharmacological treatment in infections as well as in preventing or reducing the emergence of inflammatory diseases.

• Authors: Gianandrea Guidetti, Alessandro Di Cerbo, Angela Giovazzino, Valentina Rubino, Anna Teresa Palatucci, Sara Centenaro, Elena Fraccaroli, Laura Cortese, Maria Grazia Bonomo, Giuseppina Ruggiero, Sergio Canello, Giuseppe Terrazzano
• Journal: Journal of immunology research (J Immunol Res) Vol. 2016 Pg. 5457010 ( 2016) ISSN: 2314-7156 [Electronic] Egypt
• PMID: 27597982 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Culture Media
• Cytokines
• Plant Extracts
• Interferon-gamma
• Oxytetracycline

Topics

• Adult
• Animal Feed
• Animals
• Anti-Inflammatory Agents (pharmacology)
• Cells, Cultured
• Chickens
• Culture Media (chemistry)
• Cytokines (metabolism)
• Dietary Supplements
• Dogs
• Female
• Functional Food
• Humans
• Immunomodulation (drug effects)
• Inflammation (prevention & control)
• Interferon-gamma (metabolism)
• Lymphocytes (drug effects, immunology)
• Male
• Oxytetracycline (toxicity)
• Plant Extracts (chemistry, pharmacology)
• Glycine max (chemistry)