A Single-Center Experience Comparing Alemtuzumab, Fludarabine, and Melphalan Reduced-Intensity Conditioning with Myeloablative Busulfan, Cyclophosphamide, and Antithymocyte Globulin for Chronic Granulomatous Disease.

Abstract

Myeloablative conditioning (MAC) regimens are commonly used in transplantation for chronic granulomatous disease (CGD) but are associated with toxicity. Reduced-intensity conditioning (RIC) regimens have lower toxicity but may fail to achieve stable donor chimerism. We report a comparison between 4 patients who received a RIC regimen consisting of alemtuzumab (1 mg/kg), fludarabine (150 mg/m2), and melphalan (140 mg/m2) and 14 patients who received a MAC regimen consisting of busulfan (area under the curve, 1800 to 2000 µMol/min twice daily × 4 days), cyclophosphamide (50 mg/kg/day × 4), and antithymocyte globulin (15 mg/kg twice daily on days -2 and -1, then daily on days +1 and +2). Seventy-five percent (n = 3) of RIC patients developed mixed chimerism and needed either withdrawal of immune suppression (n = 1) or additional stem cell products (n = 2) to achieve stable donor chimerism. Ninety-two percent (n = 13) of patients in the MAC group maintained >95% donor chimerism. Complications included acute graft-versus-host disease (MAC 64%, RIC 0%), chronic graft-versus-host disease (MAC 28%, RIC 0%), sinusoidal obstructive syndrome (MAC 7%, RIC 0%), bacteremia (MAC 42%, RIC 0%), fungemia (MAC 14%, RIC 0%), viral disease (MAC 7%, RIC 25%), and death (MAC 21%, RIC 0%). A RIC regimen has lower toxicity but frequently requires interventions to maintain donor chimerism compared with a MAC regimen in CGD.

• Authors: Pooja Khandelwal, Jacob J Bleesing, Stella M Davies, Rebecca A Marsh
• Journal: Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation (Biol Blood Marrow Transplant) Vol. 22 Issue 11 Pg. 2011-2018 (11 2016) ISSN: 1523-6536 [Electronic] United States
• PMID: 27543157 (Publication Type: Comparative Study, Journal Article)
• Copyright: Copyright © 2016 The American Society for Blood and Marrow Transplantation. Published by Elsevier Inc. All rights reserved.

Chemical References

• Antilymphocyte Serum
• Myeloablative Agonists
• Alemtuzumab
• Cyclophosphamide
• Vidarabine
• Busulfan
• fludarabine
• Melphalan

Topics

• Adolescent
• Alemtuzumab (administration & dosage)
• Antilymphocyte Serum (administration & dosage)
• Antineoplastic Combined Chemotherapy Protocols (therapeutic use)
• Busulfan (administration & dosage)
• Child
• Child, Preschool
• Chimerism
• Cyclophosphamide (administration & dosage)
• Female
• Granulomatous Disease, Chronic (complications, therapy)
• Hematopoietic Stem Cell Transplantation (methods)
• Humans
• Infant
• Male
• Melphalan (administration & dosage)
• Myeloablative Agonists (therapeutic use)
• Transplantation Conditioning (methods)
• Transplantation, Homologous
• Vidarabine (administration & dosage, analogs & derivatives)
• Young Adult