It has been previously demonstrated that microRNA (miR)-25 plays critical roles in collagen deposition.
Ischemia/reperfusion injury to the myocardium results in
fibrosis and collagen deposition. However, whether miR-25 is involved in the development of
hypoxia/reoxygenation (H/R)‑induced
fibrosis in cardiomyocytes or not remains largely unknown. For this purpose, in the present study, cardiomyocyte H9c2 cells were subjected to H/R. The techniques of flow cytometry, western blot analysis and RT-qPCR were used and we observed increases in the cell apoptosis rate and
fibrosis as well as blocking of the cell cycle in the G1 phase. Moreover, the expression of miR-25 was downregulated after H/R and high‑mobility group box 1 (
HMGB1) expression was increased. We also found that the overexpression of miR-25 under conditions of H/R inhibited
fibrosis and cell apoptosis as well as reversing the cell cycle blocking. Additionally, the targeting of
HMGB1 by miR-25 was confirmed by a dual‑luciferase reporter gene assay. Moreover, the effects of miR-25 were further enhanced by a transforming growth factor-β1 (TGF-β1)/Smad3 inhibitor,
SB431542, as
fibrosis was reduced and apoptosis was suppressed. In conclusion, the protective effects of miR-25 against H/R-induced
fibrosis and apoptosis H9c2 cells were due to direct targeting of
HMGB1 through the downregulation of the TGF-β1/Smad3 signaling pathway.