The effect of Nigella sativa oil on prevention of myringosclerosis in a Guinea pig model.

Abstract

OBJECTIVES:

In this study, our aim was to identify the possible effects of Nigella sativa L. (NS) [blackcumin] seed oil on the prevention of experimentally induced myringosclerosis (MS).

MATERIALS AND METHODS:

Fourteen Guinea pigs were used and they were divided into three groups. Tympanic membranes (TM) of all animals were perforated and then group I was treated with saline soaked gel foams as a control group, group II was treated with 0.5 ml NS oil soaked gel foams at 0, 24 and 48 h and group III was treated with 5 ml NS oil orally at 0, 24, 48, 72 and 120 h. After 15 days, all animals were euthanized. Tympanic membranes were evaluated macroscopically and histopathologically.

RESULTS:

Groups I showed extensive myringosclerosis in contrast to those of Groups II and III which had significantly less changes (p < 0.05). The fibrosis and inflammation in the lamina propria of the tympanic membranes of Groups I was found to be significantly more pronounced (p < 0.05). The tympanic membranes were found to be significantly thinner in Groups II and III when compared with Groups I (p < 0.05).

CONCLUSIONS:

The results of this study suggested that topical or oral administration of NS oil supressed the inflammation and fibroblastic activity in the lamina propria of the myringotomized TMs of the Guinea pigs. For providing further evidence to use plant extracts as antioxidant and antiinflammatory therapy after myringotomy or ventilation tube insertion, further clinical studies with larger population will be essential.

• Authors: Numan Kokten, Oguz Kadir Egilmez, A Isin Dogan Ekici, M Tayyar Kalcioglu, Muhammet Tekin, Erdem Yesilada
• Journal: International journal of pediatric otorhinolaryngology (Int J Pediatr Otorhinolaryngol) Vol. 88 Pg. 52-7 (Sep 2016) ISSN: 1872-8464 [Electronic] Ireland
• PMID: 27497387 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Antioxidants
• Plant Oils
• caraway oil

Topics

• Administration, Oral
• Administration, Topical
• Animals
• Anti-Inflammatory Agents (pharmacology)
• Antioxidants (pharmacology)
• Fibrosis
• Guinea Pigs
• Inflammation (pathology)
• Male
• Middle Ear Ventilation
• Mucous Membrane (drug effects, pathology)
• Myringosclerosis (pathology, prevention & control)
• Plant Oils (pharmacology)
• Tympanic Membrane (drug effects, pathology)