Abstract |
METHODS: In this study, we evaluated the pharmacokinetics, biodistribution, and dosimetry of pembrolizumab in vivo, accomplished through radiolabeling with the positron emitter 89Zr. PET imaging was used to evaluate the whole-body distribution of 89Zr-deferoxamine (Df)- pembrolizumab in two rodent models (mice and rats). Data obtained from PET scans and biodistribution studies were extrapolated to humans to estimate the dosimetry of the tracer. As a proof of concept, the biodistribution of 89Zr-Df-pembrolizumab was further investigated in a humanized murine model. RESULTS: The tracer remained stable in blood circulation throughout the study and accumulated the greatest in liver and spleen tissues. Both mice and rats showed similar biodistribution and pharmacokinetics of 89Zr-Df-pembrolizumab. In the humanized mouse model, T-cell infiltration into the salivary and lacrimal glands could be successfully visualized. CONCLUSION: These data will augment our understanding of the pharmacokinetics and biodistribution of radiolabeled pembrolizumab in vivo, while providing detailed dosimetry data that may lead to better dosing strategies in the future. These findings further demonstrate the utility of noninvasive in vivo PET imaging to dynamically track T-cell checkpoint receptor expression and localization in a humanized mouse model.
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Authors | Christopher G England, Emily B Ehlerding, Reinier Hernandez, Brian T Rekoske, Stephen A Graves, Haiyan Sun, Glenn Liu, Douglas G McNeel, Todd E Barnhart, Weibo Cai |
Journal | Journal of nuclear medicine : official publication, Society of Nuclear Medicine
(J Nucl Med)
Vol. 58
Issue 1
Pg. 162-168
(Jan 2017)
ISSN: 1535-5667 [Electronic] United States |
PMID | 27493273
(Publication Type: Journal Article)
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Copyright | © 2017 by the Society of Nuclear Medicine and Molecular Imaging. |
Chemical References |
- Antibodies, Monoclonal, Humanized
- Antineoplastic Agents
- Radioisotopes
- Radiopharmaceuticals
- Zirconium
- pembrolizumab
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Topics |
- Animals
- Antibodies, Monoclonal, Humanized
(chemistry, pharmacokinetics)
- Antineoplastic Agents
(chemistry, pharmacokinetics)
- Cell Tracking
(methods)
- Isotope Labeling
(methods)
- Male
- Metabolic Clearance Rate
- Mice
- Mice, Inbred ICR
- Organ Specificity
- Positron-Emission Tomography
(methods)
- Radioisotopes
(pharmacokinetics)
- Radiopharmaceuticals
(chemical synthesis, pharmacokinetics)
- Rats
- Rats, Sprague-Dawley
- Reproducibility of Results
- Sensitivity and Specificity
- T-Lymphocytes
(cytology, metabolism)
- Tissue Distribution
- Whole-Body Counting
- Zirconium
(pharmacokinetics)
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