Novel Antigen Detection Assay to Monitor Therapeutic Efficacy of Visceral Leishmaniasis.

Abstract

Visceral leishmaniasis (VL) diagnosis is routinely performed by invasive liver, spleen, bone marrow, or lymph node biopsies, followed by microscopic identification of the parasites. Conventional serological tests cannot distinguish active disease from asymptomatic VL or from cured infection. Here, we report the initial validation of an enzyme-linked immunosorbent assay (ELISA) assembled to detect the Leishmania infantum/donovani antigens iron superoxide dismutase 1 (Li-isd1), tryparedoxin 1 (Li-trx1), and nuclear transport factor 2 (Li-ntf2) as a tool to monitor therapeutic efficacy of VL. The assembled ELISA detected the antigens in the urine samples from seven VL patients before initiation of therapy. Importantly, the antigens were no longer detected in all patients after completion of the treatment. These preliminary observations point to a promising tool to follow treatment efficacy of VL.

• Authors: Claudia Abeijon, Om Prakash Singh, Jaya Chakravarty, Shyam Sundar, Antonio Campos-Neto
• Journal: The American journal of tropical medicine and hygiene (Am J Trop Med Hyg) Vol. 95 Issue 4 Pg. 800-802 (10 05 2016) ISSN: 1476-1645 [Electronic] United States
• PMID: 27481058 (Publication Type: Journal Article, Validation Study)
• Copyright: © The American Society of Tropical Medicine and Hygiene.

Chemical References

• Antibodies, Protozoan
• Antigens, Protozoan
• Biomarkers
• Drug Combinations
• Nucleocytoplasmic Transport Proteins
• Protozoan Proteins
• Recombinant Proteins
• tryparedoxin
• Deoxycholic Acid
• Thioredoxins
• Amphotericin B
• amphotericin B, deoxycholate drug combination
• Superoxide Dismutase

Topics

• Amphotericin B (administration & dosage)
• Animals
• Antibodies, Protozoan (immunology)
• Antigens, Protozoan (urine)
• Biomarkers (urine)
• Chickens
• Deoxycholic Acid (administration & dosage)
• Drug Combinations
• Enzyme-Linked Immunosorbent Assay (veterinary)
• Humans
• Leishmania infantum (drug effects, immunology, isolation & purification)
• Leishmaniasis, Visceral (diagnosis, drug therapy)
• Nucleocytoplasmic Transport Proteins (immunology, urine)
• Protozoan Proteins (immunology, urine)
• Rabbits
• Recombinant Proteins
• Sensitivity and Specificity
• Superoxide Dismutase (immunology, urine)
• Thioredoxins (immunology, urine)
• Treatment Outcome