HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Site-specific antibodies distinguish single amino acid substitutions in position 57 in HLA-DQ beta-chain alleles associated with insulin-dependent diabetes.

Abstract
The HLA-DQ beta-chain gene shows a close association with susceptibility or resistance to autoimmune insulin-dependent diabetes mellitus (IDDM) and it has been suggested that the amino acid in position 57 may be of pathogenetic importance. To study the expression of the IDDM associated HLA-DQ beta-chain alleles, we immunized rabbits with 12 to 13 amino acid long peptides representing HLA-DQw7 and -DQw8 allelic sequences, differing only by one amino acid in position 57 being aspartic acid (Asp) and alanine (Ala), respectively. Immunoblot analysis of lymphoblastoid cells showed that several antisera recognized a 29-kDa protein, equivalent to the expected molecular size of the HLA-DQ beta-chain to yield two antisera specific for HLA-DQw7 (pos. 57Asp) and three antisera for HLA-DQw8 (pos. 57Ala) positive cells. Analysis of HLA-DR 3/4 positive IDDM patients (n = 24) and controls (n = 19) showed that all (100%) patients were positive for pos. 57Ala antiserum compared to 13 of 19 (68%) of the controls. The remaining six controls reacted with the pos. 57Asp antisera, whereas none of the patients did. We have therefore successfully been able to generate site-specific antibodies that distinguish single amino acid substitutions in predetermined positions of allelic HLA-DQ beta-chain gene products. Such sera should become useful to detect and investigate HLA associated susceptibility to autoimmune diseases in man.
AuthorsD Atar, T Dyrberg, B Michelsen, A Karlsen, H Kofod, J Mølvig, A Lernmark
JournalJournal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 143 Issue 2 Pg. 533-8 (Jul 15 1989) ISSN: 0022-1767 [Print] United States
PMID2738402 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • HLA-DQ Antigens
  • Immune Sera
  • Peptide Fragments
Topics
  • Alleles
  • Amino Acid Sequence
  • Antibody Specificity
  • Diabetes Mellitus, Type 1 (diagnosis, genetics, immunology)
  • Enzyme-Linked Immunosorbent Assay
  • HLA-DQ Antigens (genetics, immunology)
  • Humans
  • Immune Sera
  • Immunoblotting
  • Molecular Sequence Data
  • Molecular Weight
  • Organ Specificity
  • Peptide Fragments (immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: