MZ B cells migrate in a T-bet dependent manner and might contribute to the remission of collagen-induced arthritis by the secretion of IL-10.

Abstract

In mice, marginal zone (MZ) B cells are found principally in the MZ of the spleen and characterized as CD23-negative cells, primarily express polyreactive BCRs, high levels of complement receptor-2 and TLRs. Collagen-induced arthritis (CIA) is a commonly used animal model of human rheumatoid arthritis, considered as a Th1-mediated disease. Although the importance of MZ B cells in the initiation of CIA is well established, their role in remission is unexplored. Besides, playing a central role in Th1 cell development, T-box transcription factor (T-bet) has important functions in B cells. T-bet is regulated by IFN-γ and through the BCR and TLR9, the signals that have an impact on regulatory IL-10 production. In this work, we aimed to analyze the contribution of T-bet to the function of IL-10-positive MZ B cells. We demonstrate that during the remission phase of CIA, MZ B cells express an elevated level of T-bet and confirm the existence of IL-10/T-bet coexpressing cells. Moreover, we show that T-bet-expressing MZ B cells migrate toward CXCR3 ligand and secrete IL-10 by inflammatory stimuli. Our data suggest that T-bet might contribute to the remission of CIA by facilitating the regulatory potential of IL-10-positive MZ B cells.

• Authors: Krisztina Huber, Gabriella Sármay, Dorottya Kövesdi
• Journal: European journal of immunology (Eur J Immunol) Vol. 46 Issue 9 Pg. 2239-46 (09 2016) ISSN: 1521-4141 [Electronic] Germany
• PMID: 27343199 (Publication Type: Journal Article)
• Copyright: © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Antibodies, Anti-Idiotypic
• Oligodeoxyribonucleotides
• Receptors, CXCR3
• T-Box Domain Proteins
• T-box transcription factor TBX21
• anti-IgM
• Interleukin-10
• Interferon-gamma

Topics

• Animals
• Antibodies, Anti-Idiotypic (immunology)
• Arthritis, Experimental (genetics, immunology, metabolism)
• B-Lymphocytes (drug effects, immunology, metabolism)
• Cells, Cultured
• Chemotaxis (genetics, immunology)
• Female
• Gene Expression
• Interferon-gamma (pharmacology)
• Interleukin-10 (genetics, metabolism)
• Lymphocyte Activation (immunology)
• Mice
• Oligodeoxyribonucleotides (immunology)
• Receptors, CXCR3 (metabolism)
• T-Box Domain Proteins (genetics, metabolism)