Developing a dermatological photodiagnosis system by optical image analyses and in vivo study.

Abstract

Dermatological photodynamic therapy (DPDT) involves using systematic photosensitizers in combination with light irradiation treatment to eliminate cancer cells. Therefore, a noninvasive fluorescence photodiagnosis system is critical in DPDT for diagnosing target tissues and demarcating the margin of normal tissues. This study proposes a 375-nm ring LED light module in fluorescence imaging for DPDT applications. Image reproducibility (I.R.) and image interference (I.I.) analysis were performed. The results showed that the I.R. value of this fluorescence diagnostic system was higher than 99.0%, and the I.I. from external light sources was lower than 3.0%. In addition, the result of an in vivo study showed that the Chlorin e6 red fluorescence and the scope of distribution of B16-F10 melanoma cells in a mouse ear's vein could be measured clearly using our device; however, the comparison studio with 395-nm LED lights could not focus or capture the red fluorescence effectively.

• Authors: Chung-Hsing Chang, Yu-Hsuan Lin, Cheng-Ru Li, Chun-Ming Chang, Chih-Wei Hung, Han-Chao Chang
• Journal: Photodiagnosis and photodynamic therapy (Photodiagnosis Photodyn Ther) Vol. 15 Pg. 100-4 (Sep 2016) ISSN: 1873-1597 [Electronic] Netherlands
• PMID: 27318114 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.

Chemical References

• Chlorophyllides
• Fluorescent Dyes
• Porphyrins
• phytochlorin

Topics

• Animals
• Cell Line, Tumor
• Chlorophyllides
• Dermoscopy (instrumentation, methods)
• Equipment Design
• Equipment Failure Analysis
• Fluorescent Dyes
• Lighting (instrumentation, methods)
• Melanoma (diagnostic imaging, pathology)
• Mice
• Mice, Nude
• Microscopy, Fluorescence (instrumentation, methods)
• Photography (instrumentation)
• Porphyrins
• Reproducibility of Results
• Sensitivity and Specificity
• Skin Neoplasms (diagnostic imaging, pathology)