Bromodeoxyuridine does not contribute to sister chromatid exchange events in normal or Bloom syndrome cells.

Abstract

Sister chromatid exchanges (SCEs) are considered sensitive indicators of genome instability. Detection of SCEs typically requires cells to incorporate bromodeoxyuridine (BrdU) during two rounds of DNA synthesis. Previous studies have suggested that SCEs are induced by DNA replication over BrdU-substituted DNA and that BrdU incorporation alone could be responsible for the high number of SCE events observed in cells from patients with Bloom syndrome (BS), a rare genetic disorder characterized by marked genome instability and high SCE frequency. Here we show using Strand-seq, a single cell DNA template strand sequencing technique, that the presence of variable BrdU concentrations in the cell culture medium and in DNA template strands has no effect on SCE frequency in either normal or BS cells. We conclude that BrdU does not induce SCEs and that SCEs detected in either normal or BS cells reflect DNA repair events that occur spontaneously.

• Authors: Niek van Wietmarschen, Peter M Lansdorp
• Journal: Nucleic acids research (Nucleic Acids Res) Vol. 44 Issue 14 Pg. 6787-93 (08 19 2016) ISSN: 1362-4962 [Electronic] England
• PMID: 27185886 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

Chemical References

• DNA
• Bromodeoxyuridine

Topics

• Bloom Syndrome (metabolism, pathology)
• Bromodeoxyuridine (pharmacology)
• Cell Division (drug effects)
• DNA (metabolism)
• Fibroblasts (drug effects, metabolism, pathology)
• Humans
• Lymphocytes (drug effects, metabolism, pathology)
• Sister Chromatid Exchange (drug effects)
• Templates, Genetic