[Protein Identification of Blood Nucleoprotein Complexes].

Abstract

Circulating nucleoprotein complexes were isolated-from blood plasma by affinity chromatography using immobilized polyclonal anti-histone antibodies. It was found, that the main part of DNA from histone-contained nucleoprotein complexes have size 170-180 b.p., in blood of breast cancer patients DNA with size 170-180 b.p. and DNA more then 6 k.b.p. are presented in equal quantity. Proteins from circulating nucleoprotein complexes were identified using MALDI-TOF mass-spectrometry. It was shown that nucleoprotein complexes from blood of breast cancer patients contain tumor-specific proteins, such as LDOC1L, ADP/ATP translocase 3 and Lamellipodin. These data indicate, that a part of circulating extracellular DNA have tumor origin.

• Authors: S N Tamkovich, D S Serdukov, O S Tutanov, T G Duzhak, P P Laktionov
• Journal: Bioorganicheskaia khimiia (Bioorg Khim) 2015 Nov-Dec Vol. 41 Issue 6 Pg. 686-95 ISSN: 0132-3423 [Print] Russia (Federation)
• PMID: 27125022 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Neoplasm
• Neoplasm Proteins
• Nucleoproteins

Topics

• Breast Neoplasms (blood)
• DNA, Neoplasm (blood)
• Female
• Humans
• Neoplasm Proteins (blood)
• Nucleoproteins (blood)