Abstract | BACKGROUND: Rhus verniciflua Stokes (RVS) belongs to the Anacardiaceae family and traditionally used for cancer treatment. RVS and butein, a major compound of RVS, were known to induce apoptosis via AKT inhibition in cancer cells. Thus, in this study, we investigated the effect of RVS and its derivative compounds ( fisetin, quercetin, butein) on cell death in SKOV-3/PAX cells. METHODS: RESULTS: We found that RVS and butein suppressed the growth of SKOV-3/PAX cells in a dose-dependent manner. We also found that RVS and butein produced the cleavage of caspase-9, -8, -3, and PARP. Similarly, sub-G1 phase and Annexin V-FITC positive cells were increased by RVS and butein. Moreover, RVS and butein significantly reduced AKT phosphorylation in SKOV-3/PAX cells. PI3K inhibitor LY294002 caused PARP cleavage supporting our finding. CONCLUSION: Our data clearly indicate that RVS and butein induce apoptosis of SKOV-3/PAX cells through inhibition of AKT activation. RVS and butein could be useful compounds for the treatment for paclitaxel resistant- ovarian cancer.
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Authors | Hyeong Sim Choi, Min Kyoung Kim, Youn Kyung Choi, Yong Cheol Shin, Sung-Gook Cho, Seong-Gyu Ko |
Journal | BMC complementary and alternative medicine
(BMC Complement Altern Med)
Vol. 16
Pg. 122
(Apr 27 2016)
ISSN: 1472-6882 [Electronic] England |
PMID | 27121110
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents, Phytogenic
- Chalcones
- Plant Extracts
- butein
- Proto-Oncogene Proteins c-akt
- Paclitaxel
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Topics |
- Antineoplastic Agents, Phytogenic
(pharmacology)
- Apoptosis
(drug effects)
- Cell Line, Tumor
- Chalcones
(pharmacology)
- Drug Resistance, Neoplasm
(drug effects)
- Female
- Humans
- Ovarian Neoplasms
(drug therapy)
- Paclitaxel
(pharmacology)
- Phosphorylation
- Plant Extracts
(pharmacology)
- Proto-Oncogene Proteins c-akt
(metabolism)
- Rhus
(chemistry)
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