Abstract |
In this paper, a CD44-targeted and redox-responsive drug delivery system based on mesoporous silica nanoparticles (MSNs) was synthesized by conjugating tumor-shedable hyaluronic acid (HA) on the surface of MSNs via disulfide bonds. Doxorubicin hydrochloride (DOX·HCl) was physically encapsulated into HA modified MSNs (MSNs/SS/HA@DOX) as a model drug. MSNs/SS/HA@DOX (40nm) had a high drug loading (14.1%) and redox-responsive drug release property. The cellular uptake behaviors of MSNs/SS/HA@DOX by HeLa and LO2 cells were evaluated by confocal laser scanning microscopy (CLSM) and flow cytometry (FCM). MSNs/SS/HA@DOX exhibited higher cellular uptake efficacy via CD44-mediated endocytosis by HeLa cells (CD44 over-expressed cells) than by LO2 cells (CD44 deficient cells). The in vitro cytotoxicity assay demonstrated that MSNs/SS/HA@DOX exhibited higher cytotoxicity to HeLa cells than to LO2 cells. These results indicated that MSNs/SS/HA@DOX might be promising as a multifunctional drug delivery system to improve the anti- tumor efficacy of chemotherapeutic drugs.
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Authors | Jing Zhang, Yujie Sun, Baocheng Tian, Keke Li, Lele Wang, Yan Liang, Jingtian Han |
Journal | Colloids and surfaces. B, Biointerfaces
(Colloids Surf B Biointerfaces)
Vol. 144
Pg. 293-302
(Aug 01 2016)
ISSN: 1873-4367 [Electronic] Netherlands |
PMID | 27107383
(Publication Type: Journal Article)
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Copyright | Copyright © 2016 Elsevier B.V. All rights reserved. |
Chemical References |
- Drug Carriers
- Silicon Dioxide
- Doxorubicin
- Hyaluronic Acid
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Topics |
- Cell Death
(drug effects)
- Cell Survival
(drug effects)
- Doxorubicin
(pharmacology)
- Drug Carriers
(chemistry)
- Drug Liberation
- Endocytosis
(drug effects)
- Flow Cytometry
- Fluorescence
- HeLa Cells
- Humans
- Hyaluronic Acid
(chemistry)
- Hydrogen-Ion Concentration
- Inhibitory Concentration 50
- Nanoparticles
(chemistry, ultrastructure)
- Oxidation-Reduction
- Porosity
- Silicon Dioxide
(chemistry)
- Static Electricity
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