Ultra-high performance liquid chromatography with tandem mass spectrometry method for the simultaneous quantitation of five phthalides in rat plasma: Application to a comparative pharmacokinetic study of Huo Luo Xiao Ling Dan and herb-pair extract.

Abstract

A fast, sensitive, and reliable ultra-high performance liquid chromatography with tandem mass spectrometry method has been developed and validated for the simultaneous quantitation and pharmacokinetic study of five phthalides (senkyunolide A, ligustilide, butylidenephthalide, 3-butylphthalide, and levistilide A) in rat plasma after oral administration of Huo Luo Xiao Ling Dan (HLXLD) or Angelica sinensis--Ligusticum chuanxiong herb pair (DG-CX) between normal and arthritis rats. After extraction from blood, the analytes and internal standard were subjected to ultra-high performance liquid chromatography with a Shim-pack XR-ODS column (75 × 3.0 mm(2) , 2.2 μm particles) and mobile phase was composed of methanol and water (containing 0.05% formic acid) under gradient elution conditions, with an electrospray ionization source in the positive ionization and multiple reaction monitoring mode. The lower limits of quantification were 0.192-0.800 ng/mL for all the analytes. Satisfactory linearity, precision, accuracy, mean extraction recovery, and acceptable matrix effect have been achieved. The validated method was successfully applied to a comparative pharmacokinetic study of five bioactive components in rat plasma after oral administration of HLXLD or DG-CX alone, respectively, between normal and arthritic rats. The results showed that there were unlike characters of pharmacokinetics among different groups.

• Authors: Wen Ma, Weihui Wang, Yan Peng, Qiaoxia Bian, Nannan Wang, David Y-W Lee, Ronghua Dai
• Journal: Journal of separation science (J Sep Sci) Vol. 39 Issue 11 Pg. 2057-67 (Jun 2016) ISSN: 1615-9314 [Electronic] Germany
• PMID: 27062714 (Publication Type: Comparative Study, Journal Article)
• Copyright: © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Benzofurans
• Drugs, Chinese Herbal
• Plant Extracts
• huo luo xiao ling dan
• phthalide
• Freund's Adjuvant

Topics

• Administration, Oral
• Animals
• Arthritis (chemically induced, drug therapy)
• Benzofurans (blood, chemistry)
• Chromatography, High Pressure Liquid
• Drugs, Chinese Herbal (administration & dosage, chemistry)
• Freund's Adjuvant
• Male
• Medicine, Chinese Traditional
• Molecular Structure
• Plant Extracts (administration & dosage, chemistry, pharmacokinetics)
• Rats
• Rats, Sprague-Dawley
• Tandem Mass Spectrometry