Wound healing activity of the ethanol root extract and polyphenolic rich fraction from Potentilla fulgens.

Abstract

CONTEXT:

Potentilla fulgens Wall. ex Hook (Rosaceae) is a potent medicinal plant of the Western Himalayas, where its roots are traditionally used by the local people of Uttaranchal (India) to treat wounds and tiger bites.

OBJECTIVE:

The present study scientifically evaluates the wound healing activity of P. fulgens ethanol root extract (EPF) and its ethyl acetate fraction (PFEA) on experimental rats.

MATERIALS AND METHODS:

Wounds were inflicted on animals by using both excision and incision models. The wounded animals were treated for 16 days with EPF (oral: 200-400 mg/kg and topical: 5-10% w/w) and PFEA (oral: 75 mg/kg; topical: 1.75% w/w). Various physical (wound contraction, epithelialization rate, tensile strength) and biochemical parameters (hydroxyproline, hexosamine, proteins, DNA) were examined during the study. Oxidant product (lipidperoxidase), antioxidant enzymes (catalase, superoxide-dismutase) and reduced glutathione were determined. Morphological and histopathological studies of the skin tissues were monitored.

RESULTS:

A significant (p < 0.05) wound healing property was observed when the animals were treated topically with EPF (10% w/w) and PFEA (1.75% w/w). A significantly (p < 0.05) increased in the levels of hydroxyproline, hexosamine, protein and DNA up to 59.22, 70.42, 61.01 and 60.00% was observed, respectively. This effect was further demonstrated by the morphological and histopathological representation, thus showing significant (p < 0.05) re-epethelialization on the healing area. EPF and PFEA also showed significant (p < 0.05) antioxidant activity.

CONCLUSIONS:

The present study provided the scientific evidence, where P. fulgens rich in polyphenolic components possess remarkable wound healing activities, thereby supporting the traditional claims.

• Authors: Anindita Kundu, Arka Ghosh, Narendra K Singh, Gireesh K Singh, Ankit Seth, Santosh K Maurya, Siva Hemalatha, Damiki Laloo
• Journal: Pharmaceutical biology (Pharm Biol) Vol. 54 Issue 11 Pg. 2383-2393 (Nov 2016) ISSN: 1744-5116 [Electronic] England
• PMID: 27043472 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Plant Extracts
• Polyphenols

Topics

• Animals
• Antioxidants (pharmacology)
• Female
• Male
• Plant Extracts (pharmacology, toxicity)
• Plant Roots (chemistry)
• Polyphenols (pharmacology, toxicity)
• Potentilla (chemistry)
• Rats
• Rats, Inbred Strains
• Skin (drug effects, pathology)
• Wound Healing (drug effects)