Evaluation of oral Lanzhou lamb rotavirus vaccine via passive transfusion with CD4(+)/CD8(+) T lymphocytes.

Abstract

Lanzhou Lamb derived Rotavirus (RV) Vaccine (namely LLR) for children is only used in China. Since there were no reports on evaluation of LLR, even the data of phase IV clinical trial, we proceed the evaluation of LLR through focusing on T-cell to investigate whether LLR could induce the potential function involving in protection as a vaccine. Four groups of nude mice were transfused with CD4(+)/CD8(+) T-cells isolated from LLR-immunized (primed) and LLR-unimmunized (naïve) mice via intraperitonea (i.p.) respectively. Consequently, the adoption mice were challenged with mice-origin wild rotavirus EDIM (Epizootic Diarrhea of Infant Mice) by intragastric administration. Series of fecal/serum samples were collected and viral shedding, then serum IgA/IgG and secreted IgA were assayed. Compared to the mice transfused with T lymphocytes from naïve mice, the nude mice transfused with CD4(+) T lymphocytes from primed mice induce fecal and serum IgA increasing more rapidly, and have a shorter duration of virus shedding too. Whereas, no significant difference in virus clearance was found between the mice transfused with CD8(+) T lymphocytes isolated from primed and naïve mice. Therefore, we cleared the distinct roles of transfused CD4(+)/CD8(+) T lymphocytes for rotavirus clearance in nude mice, that the viral clearance conducted by CD4(+) T lymphocytes. Meanwhile, it has ability to help induction of LLR specific immunogenicity. Comparing with the transfusion of cell from primed and naïve mice, LLR can induce CD4(+) T lymphocytes memory which is a potential index to reflect the immunogenicity and protection, while CD8(+) T lymphocytes remove rotavirus by CTL with little memory ability.

• Authors: Jialiang Du, Zhiling Lan, Yueyue Liu, Yan Liu, Qingchuan Yu, Yanchao Li, Tai Guo
• Journal: Virus research (Virus Res) Vol. 217 Pg. 101-6 (06 02 2016) ISSN: 1872-7492 [Electronic] Netherlands
• PMID: 27025573 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

Chemical References

• Immunoglobulin A
• Immunoglobulin A, Secretory
• Immunoglobulin G
• Rotavirus Vaccines

Topics

• Adoptive Transfer
• Animals
• CD4-Positive T-Lymphocytes (immunology, transplantation)
• CD8-Positive T-Lymphocytes (immunology, transplantation)
• Cell Separation
• Female
• Immunoglobulin A (blood)
• Immunoglobulin A, Secretory (blood)
• Immunoglobulin G (blood)
• Mice
• Mice, Inbred BALB C
• Rotavirus Infections
• Rotavirus Vaccines (immunology)
• Virus Shedding