B cells establish, but do not maintain, long-lived murine anti-peanut IgE(a).

Abstract

BACKGROUND:

Peanut allergy (PNA) has been reported to be transferred to tolerant recipients through organ and bone marrow (BM) transplantation. The roles T and B cells play in establishing, and the roles B cell subsets play in maintaining lifelong anti-peanut IgE levels are unknown.

OBJECTIVES:

To determine the cellular requirements for the transfer of murine PNA and to determine the role CD20(+) cells play in maintaining long-lived anti-peanut IgE levels.

METHODS:

We developed a novel adoptive transfer model to investigate the cellular requirements for transferring murine PNA. We also treated peanut-allergic (PA) mice with anti-CD20 antibody and measured IgE levels throughout treatment.

RESULTS:

Purified B220(+) cells from PA splenocytes and purified CD4(+) cells from naïve (NA) splenocytes are the minimal requirements for the adoptive transfer of PNA. Prolonged treatment of allergic mice with anti-CD20 antibody results in significant depletion of B cell subsets but does not affect anti-peanut IgE levels, symptoms, or numbers of IgE antibody secreting cells (ASCs) in the BM. Adoptive transfer of BM and spleen cells from allergic donors treated with anti-CD20 antibody does not result in the transfer of PNA in NA recipients, demonstrating that anti-CD20 antibody treatment depletes B cells capable of differentiating into peanut-specific IgE ASCs.

CONCLUSIONS AND CLINICAL RELEVANCE:

Peanut allergy can be established in a NA hosts with B220(+) cells from PA donors and CD4(+) cells from peanut-NA donors. However, long-term depletion of B220(+) cells with anti-CD20 antibody does not affect anti-peanut IgE levels. These results highlight a novel role for B cells in the development of PNA and provide evidence that long-lived anti-peanut IgE levels may be maintained by long-lived ASCs.

• Authors: D M Moutsoglou, S C Dreskin
• Journal: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology (Clin Exp Allergy) Vol. 46 Issue 4 Pg. 640-53 (Apr 2016) ISSN: 1365-2222 [Electronic] England
• PMID: 27021119 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: © 2016 John Wiley & Sons Ltd.

Chemical References

• Allergens
• Biomarkers
• Chemokine CCL2
• Immunoglobulin E
• Leukocyte Common Antigens

Topics

• Adoptive Transfer
• Allergens (immunology)
• Animals
• Arachis (adverse effects)
• B-Lymphocyte Subsets (immunology, metabolism)
• B-Lymphocytes (immunology, metabolism)
• Biomarkers
• Chemokine CCL2 (metabolism)
• Disease Models, Animal
• Immunoglobulin E (immunology)
• Immunophenotyping
• Leukocyte Common Antigens (metabolism)
• Mast Cells (immunology, metabolism)
• Mice
• Peanut Hypersensitivity (immunology, metabolism)