Abstract | BACKGROUND: METHODS: Cryo transmission electron microscopy (TEM) examined ApoA-II's influence on morphology of SMOFLipid emulsion. The influence of ApoA-II on proliferation of cancer cell lines was determined by incubating them with lipid+/- ApoA-II and anti-SR-B1 antibody. Lipid was labeled with the fluorophore, DiD, to trace lipid uptake by cancer cells in vitro by confocal microscopy and in vivo in PDAC patient derived xenograft tumours (PDXT) by fluorescence imaging. Scavenger receptor class B type-1(SR-B1) expression in PDAC cell lines and in PDAC PDXT was measured by western blotting and immunohistochemistry, respectively. RESULTS:
ApoA-II spontaneously converted lipid emulsion into very small unilamellar rHDL like vesicles (rHDL/A-II) and enhanced lipid uptake in PANC-1, CFPAC-1 and primary tumour cells as shown by confocal microscopy. SR-B1 expression was 13.2, 10.6, 3.1 and 2.3 fold higher in PANC-1, MIAPaCa-2, CFPAC-1 and BxPC3 cell lines than the normal pancreatic cell line (HPDE6) and 3.7 fold greater in PDAC tissue than in normal pancreas. ApoA-II plus lipid significantly increased the uptake of labeled lipid and promoted cell growth in PANC-1, MIAPaCa-2, CFPAC-1 and BxPC3 cells which was inhibited by anti SR-B1 antibody. Further, ApoA-II increased the uptake of lipid in xenografts by 3.4 fold. CONCLUSION:
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Authors | Sohel M Julovi, Aiqun Xue, Thao N Thanh LE, Anthony J Gill, Jerikho C Bulanadi, Mili Patel, Lynne J Waddington, Kerry-Anne Rye, Minoo J Moghaddam, Ross C Smith |
Journal | PloS one
(PLoS One)
Vol. 11
Issue 3
Pg. e0151475
( 2016)
ISSN: 1932-6203 [Electronic] United States |
PMID | 27002321
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Apolipoprotein A-II
- Lipids
- Lipoproteins, HDL
- SCARB1 protein, human
- Scavenger Receptors, Class B
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Topics |
- Apolipoprotein A-II
(metabolism)
- Carcinoma, Pancreatic Ductal
(metabolism)
- Cell Line, Tumor
- Cell Proliferation
(physiology)
- Humans
- Lipids
(physiology)
- Lipoproteins, HDL
(metabolism)
- MCF-7 Cells
- Pancreatic Neoplasms
(metabolism)
- Scavenger Receptors, Class B
(metabolism)
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