After
peripheral nerve injury, recovery of motor performance negatively correlates with the poly-innervation of neuromuscular junctions (NMJ) due to excessive sprouting of the terminal Schwann cells. Denervated muscles produce short-range diffusible sprouting stimuli, of which some are
neurotrophic factors. Based on recent data that vibrissal whisking is restored perfectly during facial nerve regeneration in blind rats from the Sprague Dawley (SD)/RCS strain, we compared the expression of
brain derived neurotrophic factor (
BDNF),
fibroblast growth factor-2 (
FGF2),
insulin growth factors 1 and 2 (IGF1, IGF2) and
nerve growth factor (
NGF) between SD/RCS and SD-rats with normal vision but poor recovery of whisking function after
facial nerve injury. To establish which trophic factors might be responsible for proper NMJ-reinnervation, the transected facial nerve was surgically repaired (facial-facial anastomosis, FFA) for subsequent analysis of
mRNA and
proteins expressed in the levator labii superioris muscle. A complicated time course of expression included (1) a late rise in
BDNF protein that followed earlier elevated gene expression, (2) an early increase in
FGF2 and IGF2
protein after 2 days with sustained gene expression, (3) reduced IGF1
protein at 28 days coincident with decline of raised
mRNA levels to baseline, and (4) reduced
NGF protein between 2 and 14 days with maintained gene expression found in blind rats but not the rats with normal vision. These findings suggest that recovery of motor function after
peripheral nerve injury is due, at least in part, to a complex regulation of lesion-associated
neurotrophic factors and
cytokines in denervated muscles. The increase of
FGF-2 protein and concomittant decrease of
NGF (with no significant changes in
BDNF or IGF levels) during the first week following FFA in SD/RCS blind rats possibly prevents the distal branching of regenerating axons resulting in reduced poly-innervation of motor endplates.