Effects of Dorzolamide on Retinal and Choroidal Blood Flow in the DBA/2J Mouse Model of Glaucoma.

Abstract

PURPOSE:

To test the hypothesis that acute topical dorzolamide (DZ) decreases intraocular pressure (IOP) and increases retinal and choroidal blood flow in the DBA/2J mouse model of glaucoma.

METHODS:

Retinal and choroidal blood flow were measured in 4- and 9-month-old DBA/2J mice, and 4-month C57BL/6 (control) mice under isoflurane anesthesia using magnetic resonance imaging. Ocular blood flow was measured at baseline, and 1 and 2 hours after topical dorzolamide. Intraocular pressure was measured using a rebound tonometer in a subset of animals at the same time points.

RESULTS:

Baseline IOP in the 4-month-old DBA/2J mice and C57BL/6 mice was not significantly different (P > 0.05), and IOP in both groups was less than in the 9-month-old DBA/2J mice (P < 0.05 for both). Compared to baseline, dorzolamide reduced IOP at 1 and 2 hours after dorzolamide in the 4- (P < 0.05) and 9-month-old (P < 0.01) DBA/2J mice, but not in the C57BL/6J mice (P > 0.05). Baseline retinal blood flow was lower in the 4-month and 9-month-old DBA/2J mice compared with the 4-month-old C57BL/6J mice (P < 0.05). Baseline choroidal blood flow in the 9-month-old DBA/2J mice was less than in the C57BL/6J mice (P < 0.05). Compared with baseline, both retinal and choroidal blood flow increased at 1-hour post-dorzolamide and remained elevated 2 hours later in the 9-month-old DBA/2J mice (P < 0.05).

CONCLUSIONS:

Dorzolamide lowers IOP and raises retinal and choroidal blood flow in older DBA/2J mice, consistent with the study hypothesis.

• Authors: Saurav Chandra, Eric R Muir, Kaiwalya Deo, Jeffrey W Kiel, Timothy Q Duong
• Journal: Investigative ophthalmology & visual science (Invest Ophthalmol Vis Sci) Vol. 57 Issue 3 Pg. 826-31 (Mar 2016) ISSN: 1552-5783 [Electronic] United States
• PMID: 26934140 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Antihypertensive Agents
• Sulfonamides
• Thiophenes
• dorzolamide

Topics

• Animals
• Antihypertensive Agents (therapeutic use)
• Blood Pressure (drug effects, physiology)
• Choroid (blood supply, drug effects, pathology)
• Disease Models, Animal
• Glaucoma (diagnosis, drug therapy, physiopathology)
• Intraocular Pressure (drug effects)
• Magnetic Resonance Imaging
• Male
• Mice
• Mice, Inbred C57BL
• Mice, Inbred DBA
• Regional Blood Flow (drug effects, physiology)
• Retina (drug effects, pathology, physiopathology)
• Sulfonamides (therapeutic use)
• Thiophenes (therapeutic use)
• Treatment Outcome